DELA1_MEDTR
ID DELA1_MEDTR Reviewed; 594 AA.
AC A0A396IUP1; A0A072V8V6;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-DEC-2018, sequence version 1.
DT 25-MAY-2022, entry version 18.
DE RecName: Full=DELLA protein 1 {ECO:0000303|PubMed:24297892, ECO:0000303|PubMed:26511916};
DE Short=MtDELLA1 {ECO:0000303|PubMed:24297892};
GN Name=DELLA1 {ECO:0000303|PubMed:24297892, ECO:0000303|PubMed:26511916};
GN OrderedLocusNames=MTR_3g065980 {ECO:0000312|EMBL:KEH34625.1};
GN ORFNames=MtrunA17_Chr3g0110971 {ECO:0000312|EMBL:RHN68173.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 60-MET--ALA-77, INDUCTION BY
RP PHOSPHATE DEPRIVATION AND GLOMUS VERSIFORME, TISSUE SPECIFICITY, AND
RP SUBCELLULAR LOCATION.
RX PubMed=24297892; DOI=10.1073/pnas.1308973110;
RA Floss D.S., Levy J.G., Levesque-Tremblay V., Pumplin N., Harrison M.J.;
RT "DELLA proteins regulate arbuscule formation in arbuscular mycorrhizal
RT symbiosis.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E5025-E5034(2013).
RN [5]
RP FUNCTION.
RX PubMed=26511916; DOI=10.1104/pp.15.01155;
RA Park H.-J., Floss D.S., Levesque-Tremblay V., Bravo A., Harrison M.J.;
RT "Hyphal branching during arbuscule development requires reduced arbuscular
RT mycorrhiza1.";
RL Plant Physiol. 169:2774-2788(2015).
CC -!- FUNCTION: Probable transcriptional regulator that acts as a repressor
CC of the gibberellin (GA) signaling pathway (By similarity). Probably
CC acts by participating in large multiprotein complexes that repress
CC transcription of GA-inducible genes (By similarity). Upon GA
CC application, it is degraded by the proteasome, allowing the GA
CC signaling pathway (By similarity). Together with DELLA2, required to
CC enable arbuscule development during arbuscular mycorrhizal (AM)
CC symbiosis with AM fungi (e.g. Glomus versiforme) via the regulation of
CC RAM1 which, in turn, regulates various AM genes (e.g. NSP1, NSP2, PT4,
CC LEC5, RAM2, EXO70I, STR and RAD1) (PubMed:26511916, PubMed:24297892).
CC {ECO:0000250|UniProtKB:Q7G7J6, ECO:0000269|PubMed:24297892,
CC ECO:0000269|PubMed:26511916}.
CC -!- SUBUNIT: May be a homodimer. {ECO:0000250|UniProtKB:Q7G7J6}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24297892}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in the vascular tissue and
CC endodermis but barely in the inner cortical cells where arbuscule are
CC formed during arbuscular mycorrhizal (AM) symbiosis.
CC {ECO:0000269|PubMed:26511916}.
CC -!- INDUCTION: Accumulates upon phosphate (Pi) deprivation
CC (PubMed:24297892). Expressed during arbuscular mycorrhizal (AM)
CC symbiosis with AM fungi (e.g. Glomus versiforme) (PubMed:24297892).
CC {ECO:0000269|PubMed:24297892}.
CC -!- DOMAIN: The DELLA motif is required for its GA-induced degradation.
CC {ECO:0000250|UniProtKB:Q7G7J6}.
CC -!- PTM: Ubiquitinated. Upon GA application it is ubiquitinated, leading to
CC its subsequent degradation. {ECO:0000250|UniProtKB:Q7G7J6}.
CC -!- DISRUPTION PHENOTYPE: The double mutant della1/della2 exhibit slender
CC shoots, early flowering and reduced root fresh weight
CC (PubMed:24297892). Impaired arbuscule formation during arbuscular
CC mycorrhizal (AM) symbiosis with AM fungi (e.g. Glomus versiforme) in
CC plant missing both DELLA1 and DELLA2 associated with a reduced
CC expression of AM genes (e.g. NSP1, NSP2, PT4 and LEC5); these
CC phenotypes are phenocopied by treatment with gibberellic acid (GA)
CC (PubMed:24297892). {ECO:0000269|PubMed:24297892}.
CC -!- SIMILARITY: Belongs to the GRAS family. DELLA subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=KEH34625.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CM001219; KEH34625.1; ALT_INIT; Genomic_DNA.
DR EMBL; PSQE01000003; RHN68173.1; -; Genomic_DNA.
DR RefSeq; XP_013460591.1; XM_013605137.1.
DR AlphaFoldDB; A0A396IUP1; -.
DR SMR; A0A396IUP1; -.
DR EnsemblPlants; KEH34625; KEH34625; MTR_3g065980.
DR GeneID; 25489240; -.
DR Gramene; KEH34625; KEH34625; MTR_3g065980.
DR KEGG; mtr:MTR_3g065980; -.
DR HOGENOM; CLU_011924_4_0_1; -.
DR OrthoDB; 559310at2759; -.
DR Proteomes; UP000002051; Chromosome 3.
DR Proteomes; UP000265566; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IEP:UniProtKB.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:1903506; P:regulation of nucleic acid-templated transcription; IMP:UniProtKB.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR Gene3D; 1.10.10.1290; -; 1.
DR InterPro; IPR038088; DELLA_N_sf.
DR InterPro; IPR030006; TF_DELLA.
DR InterPro; IPR021914; TF_DELLA_N.
DR InterPro; IPR005202; TF_GRAS.
DR PANTHER; PTHR31636; PTHR31636; 1.
DR PANTHER; PTHR31636:SF247; PTHR31636:SF247; 1.
DR Pfam; PF12041; DELLA; 1.
DR Pfam; PF03514; GRAS; 1.
DR PROSITE; PS50985; GRAS; 1.
PE 1: Evidence at protein level;
KW Gibberellin signaling pathway; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Ubl conjugation.
FT CHAIN 1..594
FT /note="DELLA protein 1"
FT /id="PRO_0000450052"
FT DOMAIN 207..587
FT /note="GRAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 214..268
FT /note="Leucine repeat I (LRI)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 216..253
FT /note="Required for possible homodimerization"
FT /evidence="ECO:0000250|UniProtKB:Q7G7J6"
FT REGION 285..350
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 364..396
FT /note="Leucine repeat II (LRII)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 406..508
FT /note="PFYRE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT REGION 511..587
FT /note="SAW"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 61..65
FT /note="DELLA motif"
FT /evidence="ECO:0000305"
FT MOTIF 221..225
FT /note="LxCxE motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 316..320
FT /note="VHIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT MOTIF 414..418
FT /note="LXXLL motif; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01191"
FT COMPBIAS 10..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 60..77
FT /note="Missing: In della1-delta18; constitutive
FT insensitivity to gibberellic acid (GA) and promotion of
FT arbuscule formation in the inner cortical cells during
FT arbuscular mycorrhizal (AM) symbiosis with AM fungi (e.g.
FT Glomus versiforme)."
FT /evidence="ECO:0000269|PubMed:24297892,
FT ECO:0000269|PubMed:26511916"
SQ SEQUENCE 594 AA; 65226 MW; B62481808B692A29 CRC64;
MKREHQESFG GGVISNNNKT NTNHLNSSKN INFGECSSMQ NTNTKQNMWR EEKETNGGGM
DELLAALGYK VRSSDMADVA QKLEQLEMVM GSAQEEGINH LSSDTVHYDP TDLYSWVQTM
LTELNPDSSQ INDPLASLGS SSEILNNTFN DDSEYDLSAI PGMAAYPPQE ENTAAKRMKT
WSEPESEPAV VMSPPPAVEN TRPVVLVDTQ ETGVRLVHTL MACAEAIQQK NLKLAEALVK
HISLLASLQT GAMRKVASYF AQALARRIYG NPEETIDSSF SEILHMHFYE SSPYLKFAHF
TANQAILEAF AGAGRVHVID FGLKQGMQWP ALMQALALRP GGPPTFRLTG IGPPQADNTD
ALQQVGWKLA QLAQTIGVQF EFRGFVCNSI ADLDPNMLEI RPGEAVAVNS VFELHTMLAR
PGSVEKVLNT VKKINPKIVT IVEQEANHNG PVFVDRFTEA LHYYSSLFDS LEGSNSSSNN
SNSNSTGLGS PSQDLLMSEI YLGKQICNVV AYEGVDRVER HETLTQWRSR MGSAGFEPVH
LGSNAFKQAS TLLALFAGGD GYRVEENNGC LMLGWHTRSL IATSAWKLPQ NESK
