DEN10_MOUSE
ID DEN10_MOUSE Reviewed; 357 AA.
AC Q9D8N2; Q3UL11; Q91WS5;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 03-NOV-2009, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=DENN domain-containing protein 10 {ECO:0000305};
DE AltName: Full=Protein FAM45A;
GN Name=Dennd10 {ECO:0000312|MGI:MGI:1915144};
GN Synonyms=Fam45a {ECO:0000312|MGI:MGI:1915144};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Liver, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) regulating
CC homeostasis of late endocytic pathway, including endosomal positioning,
CC maturation and secretion, possibly through activating Rab proteins such
CC as RAB27A and RAB27B. Seems to promote the exchange of GDP to GTP,
CC converting inactive GDP-bound RAB27A and RAB27B into their active GTP-
CC bound form. {ECO:0000250|UniProtKB:Q8TCE6}.
CC -!- SUBUNIT: Interacts with RAB27A and RAB27B (GDP-bound forms
CC preferentially). {ECO:0000250|UniProtKB:Q8TCE6}.
CC -!- SUBCELLULAR LOCATION: Late endosome {ECO:0000250|UniProtKB:Q8TCE6}.
CC -!- SIMILARITY: Belongs to the DENND10 family. {ECO:0000305}.
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DR EMBL; AK007867; BAB25318.1; -; mRNA.
DR EMBL; AK145769; BAE26640.1; -; mRNA.
DR EMBL; BC013514; AAH13514.1; -; mRNA.
DR CCDS; CCDS29942.1; -.
DR RefSeq; NP_001161301.1; NM_001167829.1.
DR RefSeq; NP_080713.2; NM_026437.3.
DR AlphaFoldDB; Q9D8N2; -.
DR STRING; 10090.ENSMUSP00000025957; -.
DR iPTMnet; Q9D8N2; -.
DR PhosphoSitePlus; Q9D8N2; -.
DR SwissPalm; Q9D8N2; -.
DR EPD; Q9D8N2; -.
DR MaxQB; Q9D8N2; -.
DR PaxDb; Q9D8N2; -.
DR PeptideAtlas; Q9D8N2; -.
DR PRIDE; Q9D8N2; -.
DR ProteomicsDB; 275837; -.
DR Antibodypedia; 49417; 49 antibodies from 14 providers.
DR DNASU; 67894; -.
DR Ensembl; ENSMUST00000025957; ENSMUSP00000025957; ENSMUSG00000024993.
DR GeneID; 67894; -.
DR KEGG; mmu:67894; -.
DR UCSC; uc008ica.2; mouse.
DR CTD; 404636; -.
DR MGI; MGI:1915144; Dennd10.
DR VEuPathDB; HostDB:ENSMUSG00000024993; -.
DR eggNOG; ENOG502QVHR; Eukaryota.
DR GeneTree; ENSGT00390000014431; -.
DR HOGENOM; CLU_050301_0_0_1; -.
DR InParanoid; Q9D8N2; -.
DR OMA; VKMMEAY; -.
DR OrthoDB; 1065142at2759; -.
DR PhylomeDB; Q9D8N2; -.
DR TreeFam; TF332501; -.
DR BioGRID-ORCS; 67894; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Fam45a; mouse.
DR PRO; PR:Q9D8N2; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q9D8N2; protein.
DR Bgee; ENSMUSG00000024993; Expressed in spermatocyte and 247 other tissues.
DR ExpressionAtlas; Q9D8N2; baseline and differential.
DR Genevisible; Q9D8N2; MM.
DR GO; GO:0005770; C:late endosome; ISS:UniProtKB.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR GO; GO:0031267; F:small GTPase binding; ISO:MGI.
DR GO; GO:0032509; P:endosome transport via multivesicular body sorting pathway; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; ISS:UniProtKB.
DR GO; GO:2000641; P:regulation of early endosome to late endosome transport; ISS:UniProtKB.
DR InterPro; IPR042431; FAM45.
DR InterPro; IPR037516; Tripartite_DENN.
DR PANTHER; PTHR28544; PTHR28544; 1.
DR PROSITE; PS50211; DENN; 1.
PE 1: Evidence at protein level;
KW Endosome; Guanine-nucleotide releasing factor; Reference proteome.
FT CHAIN 1..357
FT /note="DENN domain-containing protein 10"
FT /id="PRO_0000187036"
FT DOMAIN 1..136
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 152..299
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 301..357
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT CONFLICT 22
FT /note="S -> SS (in Ref. 2; AAH13514)"
FT /evidence="ECO:0000305"
FT CONFLICT 286
FT /note="S -> F (in Ref. 1; BAB25318)"
FT /evidence="ECO:0000305"
FT CONFLICT 308
FT /note="I -> F (in Ref. 1; BAB25318)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 357 AA; 40421 MW; DA6F38F957A8284B CRC64;
MAAVVAMDTQ LMLGVGLIEK DSNGEALWVW CYPSTTASLR NLLLRKCCLT DENKLLHPFV
FGQYRRTWFY VTTVEVPDSS VLKKVTHFSI VLTAKDFNPE KYAAFTRILC RIYLKYGSPV
KMMESYIAVL TKGICQSEEN GSFLSRDFDG RKAYLAGSIK DIVSQFGMET VILHTALMLK
KRIVVYHPKI EAVQEFTRTL PALVWHRQDW TILHSYMHLH AEELEGLQMC TGYIAGFVEL
EVSNRPDLYD VFVNLADSEI TIAPLAKEAM TMGKLHKEIG QLIVQSAEDP EKSDSQVIQD
IALKTKEIFT HLAPFSEVSD DGGKVILNVE ALKQQRFPPA TENFLYHLAA AEQMLKV
