DEN11_HUMAN
ID DEN11_HUMAN Reviewed; 455 AA.
AC A4D1U4; Q9ULS3;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=DENN domain-containing protein 11 {ECO:0000305};
DE Short=DENND11 {ECO:0000305};
DE AltName: Full=Protein LCHN;
GN Name=DENND11 {ECO:0000312|HGNC:HGNC:29472};
GN Synonyms=KIAA1147, LCHN {ECO:0000250|UniProtKB:Q0PGW2};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-455.
RC TISSUE=Brain;
RX PubMed=10574461; DOI=10.1093/dnares/6.5.329;
RA Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.;
RT "Characterization of cDNA clones selected by the GeneMark analysis from
RT size-fractionated cDNA libraries from human brain.";
RL DNA Res. 6:329-336(1999).
RN [4]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-41, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Colon carcinoma;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
RN [5]
RP ACETYLATION AT VAL-2, AND CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=25489052; DOI=10.1093/hmg/ddu611;
RA Myklebust L.M., Van Damme P., Stoeve S.I., Doerfel M.J., Abboud A.,
RA Kalvik T.V., Grauffel C., Jonckheere V., Wu Y., Swensen J., Kaasa H.,
RA Liszczak G., Marmorstein R., Reuter N., Lyon G.J., Gevaert K., Arnesen T.;
RT "Biochemical and cellular analysis of Ogden syndrome reveals downstream Nt-
RT acetylation defects.";
RL Hum. Mol. Genet. 24:1956-1976(2015).
CC -!- FUNCTION: Probable guanine nucleotide exchange factor (GEF). May
CC promote the exchange of GDP to GTP, converting inactive GDP-bound small
CC GTPases into their active GTP-bound form (Probable). May play a role in
CC neuritogenesis, as well as in neuronal recovery and/or restructuring in
CC the hippocampus following transient cerebral ischemia (By similarity).
CC {ECO:0000250|UniProtKB:Q0PGW2, ECO:0000305}.
CC -!- SIMILARITY: Belongs to the DENND11 family. {ECO:0000305}.
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DR EMBL; CH236950; EAL24019.1; -; Genomic_DNA.
DR EMBL; CH471070; EAW83974.1; -; Genomic_DNA.
DR EMBL; AB032973; BAA86461.2; -; mRNA.
DR CCDS; CCDS47726.1; -.
DR RefSeq; NP_001073861.1; NM_001080392.1.
DR AlphaFoldDB; A4D1U4; -.
DR BioGRID; 121438; 19.
DR IntAct; A4D1U4; 7.
DR STRING; 9606.ENSP00000445768; -.
DR iPTMnet; A4D1U4; -.
DR PhosphoSitePlus; A4D1U4; -.
DR BioMuta; KIAA1147; -.
DR EPD; A4D1U4; -.
DR jPOST; A4D1U4; -.
DR MassIVE; A4D1U4; -.
DR MaxQB; A4D1U4; -.
DR PaxDb; A4D1U4; -.
DR PeptideAtlas; A4D1U4; -.
DR PRIDE; A4D1U4; -.
DR ProteomicsDB; 632; -.
DR Antibodypedia; 50001; 32 antibodies from 11 providers.
DR DNASU; 57189; -.
DR Ensembl; ENST00000536163.6; ENSP00000445768.1; ENSG00000257093.7.
DR Ensembl; ENST00000575464.5; ENSP00000459381.1; ENSG00000262599.5.
DR GeneID; 57189; -.
DR KEGG; hsa:57189; -.
DR MANE-Select; ENST00000536163.6; ENSP00000445768.1; NM_001080392.2; NP_001073861.1.
DR UCSC; uc003vwk.3; human.
DR CTD; 57189; -.
DR DisGeNET; 57189; -.
DR GeneCards; DENND11; -.
DR HGNC; HGNC:29472; DENND11.
DR HPA; ENSG00000257093; Low tissue specificity.
DR MalaCards; DENND11; -.
DR neXtProt; NX_A4D1U4; -.
DR OpenTargets; ENSG00000257093; -.
DR VEuPathDB; HostDB:ENSG00000257093; -.
DR eggNOG; KOG4704; Eukaryota.
DR GeneTree; ENSGT00590000083189; -.
DR InParanoid; A4D1U4; -.
DR OMA; TGNMVEW; -.
DR OrthoDB; 1439536at2759; -.
DR PhylomeDB; A4D1U4; -.
DR TreeFam; TF329174; -.
DR PathwayCommons; A4D1U4; -.
DR SignaLink; A4D1U4; -.
DR BioGRID-ORCS; 57189; 12 hits in 1075 CRISPR screens.
DR ChiTaRS; KIAA1147; human.
DR GenomeRNAi; 57189; -.
DR Pharos; A4D1U4; Tdark.
DR PRO; PR:A4D1U4; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; A4D1U4; protein.
DR Bgee; ENSG00000257093; Expressed in corpus callosum and 99 other tissues.
DR ExpressionAtlas; A4D1U4; baseline and differential.
DR Genevisible; A4D1U4; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR InterPro; IPR018626; LCHN/Anr2.
DR InterPro; IPR037516; Tripartite_DENN.
DR Pfam; PF09804; DENND11; 1.
DR PROSITE; PS50211; DENN; 1.
PE 1: Evidence at protein level;
KW Acetylation; Guanine-nucleotide releasing factor; Methylation;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:25489052"
FT CHAIN 2..455
FT /note="DENN domain-containing protein 11"
FT /id="PRO_0000315221"
FT DOMAIN 18..187
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 214..362
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 364..455
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT REGION 1..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylvaline"
FT /evidence="ECO:0000269|PubMed:25489052"
FT MOD_RES 41
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
SQ SEQUENCE 455 AA; 51446 MW; 43449F194B74D194 CRC64;
MVEQGDAAPL LRWAEGPAVS LPQAPQPQAG GWGRGGGGGA RPAAEPPRRR EPEEPAAPEV
LLQPGRLELG DVEEDQVVAV FVVTFDPRSG NMVEWCLPQD IDLEGVEFKS MASGSHKIQS
DFIYFRKGPF FGLACFANMP VESELERGAR MKSVGILSPS YTLLYRYMHF LENQVRHQLE
MPGHYSHLAA FYEDKKGVLH AGPGRGSSLP PVYWLPSIHR YMYPEMKITH PAGCMSQFIK
FFGEQILILW KFALLRKRIL IFSPPPVGVV CYRVYCCCCL ANVSLPGIGG TIPESKPFFY
VNVADIESLE VEVSYVACTT EKIFEEKREL YDVYVDNQNV KTHHDHLQPL LKINSADREK
YRRLNEQRQM LLYSQEVEED YNPCEEDLFV LFFLEQNNRI FQTLLEVSAS QDKTLTAEHA
RGMGLDPQGD RSFLLDLLEA YGIDVMLVID NPCCP
