DEN1C_MOUSE
ID DEN1C_MOUSE Reviewed; 786 AA.
AC Q8CFK6; Q9CXF1;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=DENN domain-containing protein 1C;
DE AltName: Full=Connecdenn 3;
GN Name=Dennd1c;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate
CC RAB8A, RAB13 and RAB35. Promotes the exchange of GDP to GTP, converting
CC inactive GDP-bound Rab proteins into their active GTP-bound form.
CC {ECO:0000250|UniProtKB:Q8IV53}.
CC -!- SUBUNIT: Exhibits low nucleotide-independent RAB35-binding activity.
CC Interacts with clathrin heavy chain/CLTC and with AP2A2, but not with
CC AP2B1. {ECO:0000250|UniProtKB:Q8IV53}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q8IV53}. Cytoplasmic vesicle, clathrin-coated
CC vesicle {ECO:0000250|UniProtKB:Q8IV53}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CFK6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CFK6-2; Sequence=VSP_028091, VSP_028092;
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DR EMBL; AK014482; BAB29384.1; -; mRNA.
DR EMBL; BC035208; AAH35208.1; -; mRNA.
DR CCDS; CCDS37669.1; -. [Q8CFK6-1]
DR RefSeq; NP_705779.1; NM_153551.1. [Q8CFK6-1]
DR AlphaFoldDB; Q8CFK6; -.
DR SMR; Q8CFK6; -.
DR BioGRID; 214252; 8.
DR STRING; 10090.ENSMUSP00000011623; -.
DR iPTMnet; Q8CFK6; -.
DR PhosphoSitePlus; Q8CFK6; -.
DR EPD; Q8CFK6; -.
DR MaxQB; Q8CFK6; -.
DR PaxDb; Q8CFK6; -.
DR PeptideAtlas; Q8CFK6; -.
DR PRIDE; Q8CFK6; -.
DR ProteomicsDB; 279367; -. [Q8CFK6-1]
DR ProteomicsDB; 279368; -. [Q8CFK6-2]
DR Antibodypedia; 50064; 63 antibodies from 14 providers.
DR Ensembl; ENSMUST00000011623; ENSMUSP00000011623; ENSMUSG00000002668. [Q8CFK6-1]
DR GeneID; 70785; -.
DR KEGG; mmu:70785; -.
DR UCSC; uc008ddy.1; mouse. [Q8CFK6-1]
DR UCSC; uc008ddz.1; mouse. [Q8CFK6-2]
DR CTD; 79958; -.
DR MGI; MGI:1918035; Dennd1c.
DR VEuPathDB; HostDB:ENSMUSG00000002668; -.
DR eggNOG; KOG3569; Eukaryota.
DR GeneTree; ENSGT00940000161573; -.
DR HOGENOM; CLU_008196_4_2_1; -.
DR InParanoid; Q8CFK6; -.
DR OMA; PPILWQF; -.
DR OrthoDB; 189322at2759; -.
DR PhylomeDB; Q8CFK6; -.
DR TreeFam; TF320336; -.
DR Reactome; R-MMU-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR BioGRID-ORCS; 70785; 2 hits in 70 CRISPR screens.
DR PRO; PR:Q8CFK6; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q8CFK6; protein.
DR Bgee; ENSMUSG00000002668; Expressed in granulocyte and 83 other tissues.
DR ExpressionAtlas; Q8CFK6; baseline and differential.
DR Genevisible; Q8CFK6; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0030136; C:clathrin-coated vesicle; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR GO; GO:1901981; F:phosphatidylinositol phosphate binding; IBA:GO_Central.
DR GO; GO:0032456; P:endocytic recycling; IBA:GO_Central.
DR GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR Gene3D; 3.40.50.11500; -; 1.
DR InterPro; IPR001194; cDENN_dom.
DR InterPro; IPR005112; dDENN_dom.
DR InterPro; IPR043153; DENN_C.
DR InterPro; IPR040032; DENND1A/B/C.
DR InterPro; IPR037516; Tripartite_DENN.
DR InterPro; IPR005113; uDENN_dom.
DR PANTHER; PTHR13196; PTHR13196; 1.
DR Pfam; PF03455; dDENN; 1.
DR Pfam; PF02141; DENN; 1.
DR Pfam; PF03456; uDENN; 1.
DR SMART; SM00801; dDENN; 1.
DR SMART; SM00799; DENN; 1.
DR SMART; SM00800; uDENN; 1.
DR PROSITE; PS50211; DENN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoplasmic vesicle;
KW Guanine-nucleotide releasing factor; Phosphoprotein; Reference proteome.
FT CHAIN 1..786
FT /note="DENN domain-containing protein 1C"
FT /id="PRO_0000304677"
FT DOMAIN 13..158
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 182..318
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 320..398
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT REGION 481..553
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 653..741
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 762..786
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 401..405
FT /note="FXDXF motif"
FT /evidence="ECO:0000250|UniProtKB:Q8TEH3"
FT MOTIF 570..579
FT /note="Clathrin box"
FT /evidence="ECO:0000250|UniProtKB:Q8TEH3"
FT COMPBIAS 490..519
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 523..537
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 653..672
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 702..741
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 565
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IV53"
FT VAR_SEQ 227..267
FT /note="LTACVHASCALLYPMRWEHVLIPTLPPHLLDYCCAPMPYLI -> VRRDHFS
FT RGRDTIKPFFCSVPPLSEQSEEVAQWLQFIGSTI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028091"
FT VAR_SEQ 268..786
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_028092"
SQ SEQUENCE 786 AA; 86689 MW; 74A7FA7ABF291B6D CRC64;
MGSTETRHPP AMFDWFFEAG CPNSLEEDPP ILRQFPPDFQ EQEAMQMVPR FCFPFDIERE
PPSPAVQHFT FALTDLVGNR RFGFCRLRAG ARSCLCILSH FPWFEVFYKI LNNVGDLLAQ
NQVAEAEELL QNLQQHPLLG PRFSGRSEMD SSITVRSECG ILPPALGNSK LLSCFVAPDA
ASLPSIPENR NLTELVVAVT DENIVGLFAA LLAERRVLLT ASKLSTLTAC VHASCALLYP
MRWEHVLIPT LPPHLLDYCC APMPYLIGVH GSLAERVREK ALEDVVVLNA DSNTLETPFD
DVQALPPDVV SLLRLRLRKV ALSPGEGVSR LFLKVQALLF GGYRDALVCI PGQPVTFSEE
AFLAQKPGAP LQAFHKKAVH LQLFKQFIES RLEKLNAGEG FSDQFEQEII ACRGASSGTL
RSYQLWVDSL KKGSDALLHS MKTKTQPAVR NMYRSGDSLQ EYCASKAKSG LKGMQNLLTI
KDGDSGLQRG GSLRTPSLTS RSDRLQQRLP ISQHFGQNRP LRPSRRLKTE EGPSEPLRER
SPTLSPGDTQ NPWAEDTLDG SFLGSGEELD LLSEILDSLN VETKSGDLQR ASQSLDCCQR
GAASESCSSL PDIPVGLPWQ LEEDKRSQDP QPWSLPGDLS LLQDTPFSEV VSYSKNSCSQ
PFQQSPPSQG DPGPSLSKLD PRPSQSPCPK LLRVPTRHSP PESPQLLVST EPNSDAVQRL
QSISSPSCSH SAENPRNQPP QVLLGQACVQ PLEELGAPTY VSHVSTQQRP QDKQPRVADL
KKCFEN
