DEN2C_MOUSE
ID DEN2C_MOUSE Reviewed; 914 AA.
AC Q6P9P8; Q3T9X2; Q3UV30; Q80V46;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=DENN domain-containing protein 2C;
GN Name=Dennd2c;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Spleen, and Vagina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-261, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate
CC RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting
CC inactive GDP-bound Rab proteins into their active GTP-bound form.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6P9P8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P9P8-2; Sequence=VSP_019469, VSP_019470;
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DR EMBL; AK137637; BAE23443.1; -; mRNA.
DR EMBL; AK172232; BAE42898.1; -; mRNA.
DR EMBL; BC046440; AAH46440.1; -; mRNA.
DR EMBL; BC060666; AAH60666.1; -; mRNA.
DR AlphaFoldDB; Q6P9P8; -.
DR SMR; Q6P9P8; -.
DR STRING; 10090.ENSMUSP00000127187; -.
DR iPTMnet; Q6P9P8; -.
DR PhosphoSitePlus; Q6P9P8; -.
DR MaxQB; Q6P9P8; -.
DR PaxDb; Q6P9P8; -.
DR PRIDE; Q6P9P8; -.
DR ProteomicsDB; 279617; -. [Q6P9P8-1]
DR ProteomicsDB; 279618; -. [Q6P9P8-2]
DR UCSC; uc008qsr.1; mouse. [Q6P9P8-1]
DR MGI; MGI:3036254; Dennd2c.
DR eggNOG; KOG3569; Eukaryota.
DR InParanoid; Q6P9P8; -.
DR PhylomeDB; Q6P9P8; -.
DR Reactome; R-MMU-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR BioGRID-ORCS; 329727; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Dennd2c; mouse.
DR PRO; PR:Q6P9P8; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q6P9P8; protein.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR Gene3D; 3.40.50.11500; -; 1.
DR InterPro; IPR001194; cDENN_dom.
DR InterPro; IPR005112; dDENN_dom.
DR InterPro; IPR043153; DENN_C.
DR InterPro; IPR037516; Tripartite_DENN.
DR InterPro; IPR005113; uDENN_dom.
DR Pfam; PF03455; dDENN; 1.
DR Pfam; PF02141; DENN; 1.
DR Pfam; PF03456; uDENN; 1.
DR SMART; SM00801; dDENN; 1.
DR SMART; SM00799; DENN; 1.
DR SMART; SM00800; uDENN; 1.
DR PROSITE; PS50211; DENN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Guanine-nucleotide releasing factor; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..914
FT /note="DENN domain-containing protein 2C"
FT /id="PRO_0000242685"
FT DOMAIN 480..627
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 649..782
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 784..874
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT REGION 46..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 411..446
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..93
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 425..439
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 261
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..436
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019469"
FT VAR_SEQ 437..444
FT /note="SDAEYLPK -> MPSTCHRV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_019470"
FT CONFLICT 695
FT /note="H -> R (in Ref. 1; BAE23443)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 914 AA; 103937 MW; 8BBA622A8C04CC03 CRC64;
MDVGFTRSAV QTLSRSHCKN IKQKISQWEG RANGVTKTAK FHPKDFGVRY NCHQESPPHK
RPTGEERNGA LPRNTDVKSR DQSEDEGEGG ECRGSHPSEA GLDSSLVCAR VKQIEGCQAV
AVGPEASLPP GNFYTSQIPW RSIDGLSPDK PLNLALAPCN STGREPDLSV LDSSYGITKS
LENIYYEPEG QECGPSINPL PKPRRTFRYL SESDGIPYKE RNCDQKYCES ISCVDPSLAS
SQDPEPKKYG GKIRGRSKRK SFEFEDIQHF RNSRKIHEEL GRNAGSALYY TQSEDNIYED
IIYPAKENPY EDIPVQSFPV WRSPSAWRLP PAKSAFRTPK LPPKPQFFQR KTMELKNSQA
YFRSKITKDT TLPVTLTEWK LFRAGEVANK KKRNLPPLVL KINDTFESKR GKKRVKLQPY
TGKEAPSSKG ETSGNESDAE YLPKNRHKRL AQLQPSSKRN PHYQTLERDL IELQEQQLFE
LFVVVSLQKK PAGYTPQVIQ QFPSKGDHGY KQSKDTEERL KVIPRFCFPD SEDSAPTLEL
KSETFSFVLT GEDGSRWFGY CKKLLPEGRG KRLPEVYCMV SRLGCFNLFS KILDEVEKRR
EMSPALVYPF MRSVMEAPFP APGRTITVKS YLPGAGDESI ELCRPLDSRL EHVDFECLFK
CLSVRHLIRV CASLLLERRV IFVANSLSTL SKCGHAVVAT LYPFTWQHTY IPVLPVSMID
IVCSPTPFFI GILSCSLPHL QDLPIEEVLI VDLCADRFLQ EVSDEDEILP PKLQAALVQI
LEDRDEVLAQ EQQFSQEVTL SSLVSEAFVR FFVELVGHYS LNMTVTERGE RVFQREPFRK
SHTSRSVRHF LDLFMETQMF AGFVQDRELR QSGVKGLFEV RALQYLETIP ESEPSGVNRI
LRSLGSKMKF LHKK
