DEN2D_MOUSE
ID DEN2D_MOUSE Reviewed; 469 AA.
AC Q91VV4; Q3U028; Q9D831;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=DENN domain-containing protein 2D;
GN Name=Dennd2d;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate
CC RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting
CC inactive GDP-bound Rab proteins into their active GTP-bound form (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q91VV4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q91VV4-2; Sequence=VSP_019472;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE34027.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK008546; BAB25733.1; -; mRNA.
DR EMBL; AK157279; BAE34027.1; ALT_INIT; mRNA.
DR EMBL; BC008266; AAH08266.1; -; mRNA.
DR EMBL; BC043727; AAH43727.1; -; mRNA.
DR CCDS; CCDS51040.1; -. [Q91VV4-2]
DR RefSeq; NP_001087223.1; NM_001093754.2. [Q91VV4-2]
DR RefSeq; NP_001265870.1; NM_001278941.1.
DR AlphaFoldDB; Q91VV4; -.
DR SMR; Q91VV4; -.
DR STRING; 10090.ENSMUSP00000138462; -.
DR PhosphoSitePlus; Q91VV4; -.
DR EPD; Q91VV4; -.
DR MaxQB; Q91VV4; -.
DR PaxDb; Q91VV4; -.
DR PeptideAtlas; Q91VV4; -.
DR PRIDE; Q91VV4; -.
DR ProteomicsDB; 279193; -. [Q91VV4-1]
DR ProteomicsDB; 279194; -. [Q91VV4-2]
DR Antibodypedia; 53750; 78 antibodies from 12 providers.
DR DNASU; 72121; -.
DR Ensembl; ENSMUST00000029508; ENSMUSP00000029508; ENSMUSG00000027901. [Q91VV4-2]
DR GeneID; 72121; -.
DR KEGG; mmu:72121; -.
DR UCSC; uc008qwa.2; mouse. [Q91VV4-2]
DR UCSC; uc012cvr.2; mouse. [Q91VV4-1]
DR CTD; 79961; -.
DR MGI; MGI:2181193; Dennd2d.
DR VEuPathDB; HostDB:ENSMUSG00000027901; -.
DR eggNOG; KOG3569; Eukaryota.
DR GeneTree; ENSGT00950000182931; -.
DR InParanoid; Q91VV4; -.
DR PhylomeDB; Q91VV4; -.
DR TreeFam; TF320336; -.
DR Reactome; R-MMU-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR BioGRID-ORCS; 72121; 6 hits in 69 CRISPR screens.
DR PRO; PR:Q91VV4; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q91VV4; protein.
DR Bgee; ENSMUSG00000027901; Expressed in granulocyte and 127 other tissues.
DR ExpressionAtlas; Q91VV4; baseline and differential.
DR Genevisible; Q91VV4; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR Gene3D; 3.40.50.11500; -; 1.
DR InterPro; IPR001194; cDENN_dom.
DR InterPro; IPR005112; dDENN_dom.
DR InterPro; IPR043153; DENN_C.
DR InterPro; IPR037516; Tripartite_DENN.
DR InterPro; IPR005113; uDENN_dom.
DR Pfam; PF03455; dDENN; 1.
DR Pfam; PF02141; DENN; 1.
DR Pfam; PF03456; uDENN; 1.
DR SMART; SM00801; dDENN; 1.
DR SMART; SM00799; DENN; 1.
DR SMART; SM00800; uDENN; 1.
DR PROSITE; PS50211; DENN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Guanine-nucleotide releasing factor;
KW Reference proteome.
FT CHAIN 1..469
FT /note="DENN domain-containing protein 2D"
FT /id="PRO_0000242687"
FT DOMAIN 55..204
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 226..359
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 361..445
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT REGION 17..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..22
FT /note="MEGQGVGRTLRLLRNRLPRLRA -> MDGLGRRLRASLRLKRGQR (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_019472"
FT CONFLICT 66
FT /note="R -> H (in Ref. 1; BAE34027)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 469 AA; 53211 MW; 1EEE763261AFEE35 CRC64;
MEGQGVGRTL RLLRNRLPRL RAGQSQNNPG EAVTEPERIQ EHSPSSFAGG QHFFEYLLVV
SLKKKRLGDD YEPTITYQFP KRENLLRGQQ EEEDRLLSAI PLFCFPDGNE WAPLTEYPRE
TFSFVLTNVD GSRKIGYCRR LLPAGPGPQL PKVYCIISCI GCFGLFSKIL DEVEKRHQIS
MAVIYPFMQG LREAAFPAPG KTVTLKSFIP DSGTEFISLT RPLDSHLEHV DFSVLLHCLH
LEQIIQIFAS AVLERKIIFL AEGLSTLSQC IHAAAALLYP FSWAHTYIPV VPESLLATVC
CPTPFMVGVQ MRFLQEVMDS PMEEVLLVNL CEGTFLLSVG DEKDILPPKL QDDILDSLGQ
GINELKTSEQ INEHVSGPFV QFFVKTVGHY ASYIKREASG QGHFQERSFC KAVTSKTKRR
FVKKFVKTQL FSLFIQEAEK SRNPPAGYFQ KKILEYEEQK KQKKSRERL
