DEN5A_RAT
ID DEN5A_RAT Reviewed; 1287 AA.
AC G3V7Q0;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=DENN domain-containing protein 5A;
DE AltName: Full=Rab6-interacting protein 1;
DE Short=Rab6IP1;
GN Name=Dennd5a;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15632090; DOI=10.1101/gr.2889405;
RA Florea L., Di Francesco V., Miller J., Turner R., Yao A., Harris M.,
RA Walenz B., Mobarry C., Merkulov G.V., Charlab R., Dew I., Deng Z.,
RA Istrail S., Li P., Sutton G.;
RT "Gene and alternative splicing annotation with AIR.";
RL Genome Res. 15:54-66(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=27866705; DOI=10.1016/j.ajhg.2016.10.006;
RA Han C., Alkhater R., Froukh T., Minassian A.G., Galati M., Liu R.H.,
RA Fotouhi M., Sommerfeld J., Alfrook A.J., Marshall C., Walker S., Bauer P.,
RA Scherer S.W., Riess O., Buchert R., Minassian B.A., McPherson P.S.;
RT "Epileptic encephalopathy caused by mutations in the guanine nucleotide
RT exchange factor DENND5A.";
RL Am. J. Hum. Genet. 99:1359-1367(2016).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate
CC RAB6A and RAB39A and/or RAB39B. Promotes the exchange of GDP to GTP,
CC converting inactive GDP-bound Rab proteins into their active GTP-bound
CC form (By similarity). Involved in the negative regulation of neurite
CC outgrowth (PubMed:27866705). {ECO:0000250|UniProtKB:Q6IQ26,
CC ECO:0000269|PubMed:27866705}.
CC -!- SUBUNIT: Interacts with RAB6A bound to GTP.
CC {ECO:0000250|UniProtKB:Q6PAL8}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q6PAL8}.
CC -!- TISSUE SPECIFICITY: Expressed in developing brain and developing
CC neurons. {ECO:0000269|PubMed:27866705}.
CC -!- DEVELOPMENTAL STAGE: In the brain, expression is high at embryonic day
CC 18 and continually decreases through to postnatal day 60.
CC {ECO:0000269|PubMed:27866705}.
CC -!- SIMILARITY: Belongs to the RAB6IP1 family. {ECO:0000305}.
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DR EMBL; AABR07071965; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC098265; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH473956; EDM17883.1; -; Genomic_DNA.
DR RefSeq; NP_001101016.2; NM_001107546.2.
DR AlphaFoldDB; G3V7Q0; -.
DR SMR; G3V7Q0; -.
DR STRING; 10116.ENSRNOP00000017485; -.
DR iPTMnet; G3V7Q0; -.
DR PhosphoSitePlus; G3V7Q0; -.
DR PaxDb; G3V7Q0; -.
DR PRIDE; G3V7Q0; -.
DR Ensembl; ENSRNOT00000017485; ENSRNOP00000017485; ENSRNOG00000012206.
DR GeneID; 308942; -.
DR KEGG; rno:308942; -.
DR CTD; 23258; -.
DR RGD; 1306759; Dennd5a.
DR eggNOG; KOG2080; Eukaryota.
DR GeneTree; ENSGT00940000153678; -.
DR HOGENOM; CLU_004201_2_0_1; -.
DR InParanoid; G3V7Q0; -.
DR OMA; KVTQEMY; -.
DR OrthoDB; 53600at2759; -.
DR TreeFam; TF313237; -.
DR Reactome; R-RNO-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR PRO; PR:G3V7Q0; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Proteomes; UP000234681; Chromosome 1.
DR Bgee; ENSRNOG00000012206; Expressed in brain and 20 other tissues.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005794; C:Golgi apparatus; ISO:RGD.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030904; C:retromer complex; IEA:Ensembl.
DR GO; GO:0005802; C:trans-Golgi network; ISO:RGD.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISO:RGD.
DR GO; GO:0031267; F:small GTPase binding; ISO:RGD.
DR GO; GO:0010977; P:negative regulation of neuron projection development; IMP:UniProtKB.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISO:RGD.
DR Gene3D; 1.20.58.900; -; 3.
DR Gene3D; 3.40.50.11500; -; 1.
DR InterPro; IPR001194; cDENN_dom.
DR InterPro; IPR005112; dDENN_dom.
DR InterPro; IPR043153; DENN_C.
DR InterPro; IPR001024; PLAT/LH2_dom.
DR InterPro; IPR036392; PLAT/LH2_dom_sf.
DR InterPro; IPR004012; Run_dom.
DR InterPro; IPR037213; Run_dom_sf.
DR InterPro; IPR037516; Tripartite_DENN.
DR InterPro; IPR005113; uDENN_dom.
DR Pfam; PF03455; dDENN; 1.
DR Pfam; PF02141; DENN; 1.
DR Pfam; PF01477; PLAT; 1.
DR Pfam; PF02759; RUN; 2.
DR Pfam; PF03456; uDENN; 1.
DR SMART; SM00801; dDENN; 1.
DR SMART; SM00799; DENN; 1.
DR SMART; SM00593; RUN; 2.
DR SMART; SM00800; uDENN; 1.
DR SUPFAM; SSF140741; SSF140741; 2.
DR SUPFAM; SSF49723; SSF49723; 1.
DR PROSITE; PS50211; DENN; 1.
DR PROSITE; PS50095; PLAT; 1.
DR PROSITE; PS50826; RUN; 2.
PE 2: Evidence at transcript level;
KW Golgi apparatus; Guanine-nucleotide releasing factor; Membrane;
KW Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..1287
FT /note="DENN domain-containing protein 5A"
FT /id="PRO_0000440130"
FT DOMAIN 57..259
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 278..414
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 416..598
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 787..950
FT /note="RUN 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00178"
FT DOMAIN 954..1062
FT /note="PLAT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00152"
FT DOMAIN 1134..1280
FT /note="RUN 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00178"
FT MOD_RES 193
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6IQ26"
FT MOD_RES 1079
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q6PAL8"
FT MOD_RES 1085
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6PAL8"
FT MOD_RES 1087
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6IQ26"
FT MOD_RES 1096
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6IQ26"
SQ SEQUENCE 1287 AA; 146637 MW; 68AA8962F02B643B CRC64;
MSGGGGGGGS APSRFADYFV ICGLDTETGL EPDELSALCQ YIQASKARDG ASPFISSTTE
GENFEQTPLR RTFKSKVLAR YPENVDWNPF DQDAVGMLCM PKGLAFKTQA DPREPQFHAF
IITREDGSRT FGFALTFYEE VTSKQICSAM QTLYHMHNAE YDVLHAPLAD GGDQSGMEDG
EGIPGTKLQR FNSYDISRDT LYVSKCICLI TPMSFMKACR SVLQQLHQAV TSPQPPPLPL
ESYIYNVLYE VPLPPPGRSL KFSGVYGPII CQRPSTNELP LFDFPVKEVF ELLGVENVFQ
LFTCALLEFQ ILLYSQHYQR LMTVAETITA LMFPFQWQHV YVPILPASLL HFLDAPVPYL
MGLHSNGLDD RSKLELPQEA NLCFVDVDNH FIELPEDLPQ FPNKLEFVQE VSEILMAFGV
PPEGNLHCSE SASKLKRIRA SELVSDKKNG NIAGSPLHSY ELLKENETIA RLQALVKRTG
VSLEKLEVRE DPSSNKDFKV QCDEEELRIY QLNIQIREVF ANRFTQMFAD YEVFVIQPSQ
DKESWLSNRE QMQNFDKASF LSDQPEPYLP FLSRFLETQM FASFIDNKIM CHDDDDKDPV
LRVFDSRVDK IRLLNVRTPT LRTSMYQKCT TVDEAEKAIE LRLAKIDHTA VHPHLLDMKI
GQGKYEPGFF PKLQSDVLCT GPASNKWTKR NAPAQWRRKD RQKQHTEHLR LDNDQREKYI
QEARNMGSTI RQPKLSNLSP SVIAQTNWKF VEGLLKECRN KTKRMLVEKM GREAVELGHG
EVNITGVEEN TLIASLCDLL ERIWSHGLQV KQGKSALWSH LLHYQENRQR KLTSGSLSTS
GILLDSERRK SDASAVMSPL RVSLIQDMRH IQNIGEIKTD VGKARAWVRL SMEKKLLSRH
LKQLLSDHEL TKKLYKRYAF LRCDDEKEQF LYHLLSFNAV DYFCFTNVFT TILIPYHILI
VPSKKLGGSM FTANPWICIS GELGETQILQ IPRNVLEMTF ECQNLGKLTT VQIGHDNSGL
YAKWLVEYVM VRNEITGHTY KFPCGRWLGK GMDDGSLERV LVGELLTSLP EVDERPCRTP
PLQQSPSVIR RLVTISPNNK PKLNTGQIQE SIGEAVNGIV KHFHKPEKER GSLTLLLCGE
CGLVSALEQA FQHGFKSPRL FKNVFIWDFL EKAQTYYETL EQNDVVPEEN WHTRARNFCR
FVTAVNNTPR NIGKDGKFQM LVCLGARDHL LHHWIALLAD CPITAHMYED VALIKDHTLV
NSLIRVLQTL QEFNITLDTS LVKGIDI
