DEN5B_DANRE
ID DEN5B_DANRE Reviewed; 1311 AA.
AC Q6NXD8; Q5RJ77;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=DENN domain-containing protein 5B;
DE AltName: Full=Rab6IP1-like protein;
GN Name=dennd5b; ORFNames=si:ch211-11c20.2, zgc:77218;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) which may activate
CC the small GTPases Rab. May promote the exchange of GDP to GTP,
CC converting inactive GDP-bound Rab proteins into their active GTP-bound
CC form (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RAB6IP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAI11568.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; BX001012; CAI11568.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC067136; AAH67136.1; -; mRNA.
DR RefSeq; NP_997809.1; NM_212644.1.
DR AlphaFoldDB; Q6NXD8; -.
DR SMR; Q6NXD8; -.
DR STRING; 7955.ENSDARP00000073917; -.
DR PaxDb; Q6NXD8; -.
DR PRIDE; Q6NXD8; -.
DR Ensembl; ENSDART00000033053; ENSDARP00000032722; ENSDARG00000003789.
DR GeneID; 323528; -.
DR KEGG; dre:323528; -.
DR CTD; 160518; -.
DR ZFIN; ZDB-GENE-030131-2248; dennd5b.
DR eggNOG; KOG2080; Eukaryota.
DR GeneTree; ENSGT00940000153678; -.
DR InParanoid; Q6NXD8; -.
DR OMA; NTIHMYE; -.
DR PhylomeDB; Q6NXD8; -.
DR PRO; PR:Q6NXD8; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 4.
DR Bgee; ENSDARG00000003789; Expressed in retina and 29 other tissues.
DR ExpressionAtlas; Q6NXD8; differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR GO; GO:0031267; F:small GTPase binding; IBA:GO_Central.
DR Gene3D; 1.20.58.900; -; 3.
DR Gene3D; 3.40.50.11500; -; 1.
DR InterPro; IPR001194; cDENN_dom.
DR InterPro; IPR005112; dDENN_dom.
DR InterPro; IPR043153; DENN_C.
DR InterPro; IPR001024; PLAT/LH2_dom.
DR InterPro; IPR036392; PLAT/LH2_dom_sf.
DR InterPro; IPR004012; Run_dom.
DR InterPro; IPR037213; Run_dom_sf.
DR InterPro; IPR037516; Tripartite_DENN.
DR InterPro; IPR005113; uDENN_dom.
DR Pfam; PF03455; dDENN; 1.
DR Pfam; PF02141; DENN; 1.
DR Pfam; PF01477; PLAT; 1.
DR Pfam; PF02759; RUN; 2.
DR Pfam; PF03456; uDENN; 1.
DR SMART; SM00801; dDENN; 1.
DR SMART; SM00799; DENN; 1.
DR SMART; SM00593; RUN; 2.
DR SMART; SM00800; uDENN; 1.
DR SUPFAM; SSF140741; SSF140741; 2.
DR SUPFAM; SSF49723; SSF49723; 1.
DR PROSITE; PS50211; DENN; 1.
DR PROSITE; PS50095; PLAT; 1.
DR PROSITE; PS50826; RUN; 2.
PE 2: Evidence at transcript level;
KW Guanine-nucleotide releasing factor; Membrane; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..1311
FT /note="DENN domain-containing protein 5B"
FT /id="PRO_0000326533"
FT TRANSMEM 962..982
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 53..270
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 289..452
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 454..619
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 809..969
FT /note="RUN 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00178"
FT DOMAIN 973..1081
FT /note="PLAT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00152"
FT DOMAIN 1155..1306
FT /note="RUN 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00178"
FT REGION 154..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 854..874
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 154..191
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 140
FT /note="P -> L (in Ref. 2; AAH67136)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="H -> N (in Ref. 2; AAH67136)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1311 AA; 147701 MW; 1DCBC20FF52F8FEC CRC64;
MSATMSGSGS APCRFAHYFI VCGLDDETGL EPDALAALYQ WLEADRQGKD PEATAAGENF
DQSPLKRTFK SKVLAHYPEN IECNPFDQDA VNMLCMPKGL SFRTQRDSLA PHFHSFLITR
EDGSRTYGFV HTFYEEVTSP QICSAMQTLH QMHQAEHNTS AQNCTSSSSS SSSSSSSSSM
DSLSSSLDDV DSPSAHGGRR TCEGYDSIRD TLYVSKAMCL IAPMPFMHAC KRFLAQMHRA
VSCSPPPPLP LESYIYNVLY EVPLPASGRS LKFHGVYEPI LCQRPGVGEL PLADFPLADA
FRLLGVENLV QLFTCVLLEM QILLYSQDYQ RLMVVAEGIT TLLFPFQWQH VYVPILPASL
LHFLDAPVPY LMGLQSKEGT DRSKLELPQE ANLCFVDIDN HCIELPEDFP QFPNRSEFIQ
ELSEVLLSFG QSPEGGSSSP EPAVMSLQTS VLEKELKSTS LRELVDDKTN GNLGGEALDV
LELLQGNPTL ERLQALAKRT GVKVARLEAL AAGQKGDEGV GGRSPVEEEE LRNAKLNVQL
REVFAARFAT MFADYESFVI QNSPDLESWL TNREQMHNFD KASFLSDQPE PYLPFLSHFI
ETQMFATFID NKIMSQWEEK EPLLRVFDGR IEKARLYNVR APSLRSSVYQ KCTFLKESAQ
AIEQRLRKID HTAIHPHLLD MKIGQGKYQQ GFFPKLQADV LASGPTSNKW SNRTCSTQRR
VERHRQQNEH LVLDNELKEK YMQEARNLGK NLRQPKLSDL SPAVIAQTNW KFVEGLLKEC
KMKTKRMLVE KMGREAVELG HGEANITGLE ENTLIASLCD LLERIWSHGL QVKQGKSALW
SHLLHYQARE EKNEQQLESP VSNGQERRKT ESSVGLPGLR VSVIQDMRHI QNMAEIKTDV
GRARAWIRLS LEKKLLSQHL KQLLSNQALT KKLYKRYAFL RCEEEKEQFL FHLLSLNAVD
YFCFTSVFTT IMIPYRAVII PIKKLSNAMT TSNPWLCVSG ELGDSGILQI TKNILEMTFD
CQNLGKLTTV QLGHDNSGLL AKWLVDCVMV RNEITGHTYK FPCGRWLGKG VDDGSLERVL
IGEFVVPCGD DEGGRGSKTP PLQRSPSQIR RISITSLTGK GNKPTTVQIQ ESIGEAVNNI
IKHFHKPEKE RGSLTVLLCG EGGLVWALEQ FFHHGFRSAR IFQKNVFVWD FFERIVAFME
NRDQTGDLQE SPEPLSLNSE SLCRYVNAIN NMPRNIGKDG KFQLLVCLGA RDRLLPQWLP
LLAESPVITR MYEENALLRD KLTVSSLLGV LETLHDFPIT LETSLTKSVE L
