DEN6B_HUMAN
ID DEN6B_HUMAN Reviewed; 585 AA.
AC Q8NEG7; A6X8I5;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Protein DENND6B;
DE AltName: Full=DENN domain-containing protein 6B;
GN Name=DENND6B; Synonyms=FAM116B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10591208; DOI=10.1038/990031;
RA Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M.,
RA Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C.,
RA Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E.,
RA Bridgeman A.M., Buck D., Burgess J., Burrill W.D., Burton J., Carder C.,
RA Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G.,
RA Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V.,
RA Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M.,
RA Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A.,
RA Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C.,
RA Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E.,
RA Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F.,
RA Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M.,
RA Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A.,
RA Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D.,
RA Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y.,
RA Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S.,
RA Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E.,
RA Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L.,
RA Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L.,
RA Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N.,
RA Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A.,
RA Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L.,
RA Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P.,
RA Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P.,
RA Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q.,
RA Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J.,
RA Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J.,
RA Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D.,
RA Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T.,
RA Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P.,
RA Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K.,
RA Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R.,
RA Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L.,
RA McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J.,
RA Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E.,
RA Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P.,
RA Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y.,
RA Wright H.;
RT "The DNA sequence of human chromosome 22.";
RL Nature 402:489-495(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION.
RX PubMed=21330364; DOI=10.1074/jbc.r110.217067;
RA Marat A.L., Dokainish H., McPherson P.S.;
RT "DENN domain proteins: regulators of Rab GTPases.";
RL J. Biol. Chem. 286:13791-13800(2011).
RN [4]
RP FUNCTION.
RX PubMed=22595670; DOI=10.1016/j.devcel.2012.04.010;
RA Linford A., Yoshimura S., Nunes Bastos R., Langemeyer L., Gerondopoulos A.,
RA Rigden D.J., Barr F.A.;
RT "Rab14 and its exchange factor FAM116 link endocytic recycling and adherens
RT junction stability in migrating cells.";
RL Dev. Cell 22:952-966(2012).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) for RAB14. Also has
CC some, lesser GEF activity towards RAB35. {ECO:0000269|PubMed:22595670}.
CC -!- SUBCELLULAR LOCATION: Recycling endosome {ECO:0000305}. Cytoplasm
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the DENND6 family. {ECO:0000305}.
CC -!- CAUTION: Identified as having similarity to the core DENN family and
CC referred to as DENN6B (PubMed:21330364). Prediction methods do not
CC indicate a DENN domain for this sequence and, the exact role of the
CC DENN or this DENN-like domain in GEF activity needs to be clarified.
CC {ECO:0000305|PubMed:21330364}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CT009622; CAO03304.1; -; Genomic_DNA.
DR EMBL; CR559946; CAO03304.1; JOINED; Genomic_DNA.
DR EMBL; CR759740; CAO03304.1; JOINED; Genomic_DNA.
DR EMBL; CR932343; CAO03304.1; JOINED; Genomic_DNA.
DR EMBL; CR932344; CAO03304.1; JOINED; Genomic_DNA.
DR EMBL; CR932344; CAO03484.1; -; Genomic_DNA.
DR EMBL; CR559946; CAO03484.1; JOINED; Genomic_DNA.
DR EMBL; CR759740; CAO03484.1; JOINED; Genomic_DNA.
DR EMBL; CR932343; CAO03484.1; JOINED; Genomic_DNA.
DR EMBL; CT009622; CAO03484.1; JOINED; Genomic_DNA.
DR EMBL; CR559946; CAO03525.1; -; Genomic_DNA.
DR EMBL; CR759740; CAO03525.1; JOINED; Genomic_DNA.
DR EMBL; CR932343; CAO03525.1; JOINED; Genomic_DNA.
DR EMBL; CR932344; CAO03525.1; JOINED; Genomic_DNA.
DR EMBL; CT009622; CAO03525.1; JOINED; Genomic_DNA.
DR EMBL; CR932343; CAO03579.1; -; Genomic_DNA.
DR EMBL; CR559946; CAO03579.1; JOINED; Genomic_DNA.
DR EMBL; CR759740; CAO03579.1; JOINED; Genomic_DNA.
DR EMBL; CR932344; CAO03579.1; JOINED; Genomic_DNA.
DR EMBL; CT009622; CAO03579.1; JOINED; Genomic_DNA.
DR EMBL; CR759740; CAO03621.1; -; Genomic_DNA.
DR EMBL; CR559946; CAO03621.1; JOINED; Genomic_DNA.
DR EMBL; CR932343; CAO03621.1; JOINED; Genomic_DNA.
DR EMBL; CR932344; CAO03621.1; JOINED; Genomic_DNA.
DR EMBL; CT009622; CAO03621.1; JOINED; Genomic_DNA.
DR EMBL; BC031069; AAH31069.1; -; mRNA.
DR CCDS; CCDS46732.1; -.
DR RefSeq; NP_001001794.3; NM_001001794.3.
DR AlphaFoldDB; Q8NEG7; -.
DR BioGRID; 136098; 6.
DR IntAct; Q8NEG7; 7.
DR STRING; 9606.ENSP00000391524; -.
DR iPTMnet; Q8NEG7; -.
DR PhosphoSitePlus; Q8NEG7; -.
DR BioMuta; DENND6B; -.
DR DMDM; 74760209; -.
DR EPD; Q8NEG7; -.
DR jPOST; Q8NEG7; -.
DR MassIVE; Q8NEG7; -.
DR MaxQB; Q8NEG7; -.
DR PaxDb; Q8NEG7; -.
DR PeptideAtlas; Q8NEG7; -.
DR PRIDE; Q8NEG7; -.
DR ProteomicsDB; 73165; -.
DR Antibodypedia; 6832; 49 antibodies from 16 providers.
DR DNASU; 414918; -.
DR Ensembl; ENST00000413817.8; ENSP00000391524.2; ENSG00000205593.12.
DR GeneID; 414918; -.
DR KEGG; hsa:414918; -.
DR MANE-Select; ENST00000413817.8; ENSP00000391524.2; NM_001001794.4; NP_001001794.3.
DR UCSC; uc011arv.2; human.
DR CTD; 414918; -.
DR DisGeNET; 414918; -.
DR GeneCards; DENND6B; -.
DR HGNC; HGNC:32690; DENND6B.
DR HPA; ENSG00000205593; Low tissue specificity.
DR neXtProt; NX_Q8NEG7; -.
DR OpenTargets; ENSG00000205593; -.
DR PharmGKB; PA144596432; -.
DR VEuPathDB; HostDB:ENSG00000205593; -.
DR eggNOG; KOG2432; Eukaryota.
DR GeneTree; ENSGT00390000005529; -.
DR HOGENOM; CLU_017013_0_1_1; -.
DR InParanoid; Q8NEG7; -.
DR OMA; QLVYPYD; -.
DR OrthoDB; 252077at2759; -.
DR PhylomeDB; Q8NEG7; -.
DR TreeFam; TF320228; -.
DR PathwayCommons; Q8NEG7; -.
DR Reactome; R-HSA-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR SignaLink; Q8NEG7; -.
DR BioGRID-ORCS; 414918; 12 hits in 1079 CRISPR screens.
DR GenomeRNAi; 414918; -.
DR Pharos; Q8NEG7; Tbio.
DR PRO; PR:Q8NEG7; -.
DR Proteomes; UP000005640; Chromosome 22.
DR RNAct; Q8NEG7; protein.
DR Bgee; ENSG00000205593; Expressed in right uterine tube and 109 other tissues.
DR ExpressionAtlas; Q8NEG7; baseline and differential.
DR Genevisible; Q8NEG7; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0055037; C:recycling endosome; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:UniProtKB.
DR InterPro; IPR018307; ABL9/DENND6_dom.
DR InterPro; IPR024224; DENND6.
DR InterPro; IPR037516; Tripartite_DENN.
DR PANTHER; PTHR13677; PTHR13677; 1.
DR Pfam; PF09794; Avl9; 1.
DR PROSITE; PS50211; DENN; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Endosome; Guanine-nucleotide releasing factor;
KW Reference proteome.
FT CHAIN 1..585
FT /note="Protein DENND6B"
FT /id="PRO_0000264990"
FT DOMAIN 43..214
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 246..373
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 375..499
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
SQ SEQUENCE 585 AA; 66464 MW; 2B2D5F46647D448C CRC64;
MDALLGTGPR RARGCLGAAG PTSSGRAART PAAPWARFSA WLECVCVVTF DLELGQALEL
VYPNDFRLTD KEKSSICYLS FPDSHSGCLG DTQFSFRMRQ CGGQRSPWHA DDRHYNSRAP
VALQREPAHY FGYVYFRQVK DSSVKRGYFQ KSLVLVSRLP FVRLFQALLS LIAPEYFDKL
APCLEAVCSE IDQWPAPAPG QTLNLPVMGV VVQVRIPSRV DKSESSPPKQ FDQENLLPAP
VVLASVHELD LFRCFRPVLT HMQTLWELML LGEPLLVLAP SPDVSSEMVL ALTSCLQPLR
FCCDFRPYFT IHDSEFKEFT TRTQAPPNVV LGVTNPFFIK TLQHWPHILR VGEPKMSGDL
PKQVKLKKPS RLKTLDTKPG LYTAYTAHLH RDKALLKRLL KGVQKKRPSD VQSALLRRHL
LELTQSFIIP LEHYMASLMP LQKSITPWKT PPQIQPFSQD DFLRSLEHAG PQLTCILKGD
WLGLYRRFFK SPHFDGWYRQ RHKEMALKLE ALHLEAICEA NIETWMKDKS EVEVVDLVLK
LREKLVRAQG HQLPVKEATL QRAQLYIETV IGSLPKDLQA VLCPP
