DEN6B_MOUSE
ID DEN6B_MOUSE Reviewed; 585 AA.
AC Q9D9V7; B2RSU3;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein DENND6B;
DE AltName: Full=DENN domain-containing protein 6B;
GN Name=Dennd6b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Dendritic cell, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION.
RX PubMed=21330364; DOI=10.1074/jbc.r110.217067;
RA Marat A.L., Dokainish H., McPherson P.S.;
RT "DENN domain proteins: regulators of Rab GTPases.";
RL J. Biol. Chem. 286:13791-13800(2011).
CC -!- FUNCTION: Guanine nucleotide exchange factor (GEF) for RAB14. Also has
CC some, lesser GEF activity towards RAB35 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Recycling endosome {ECO:0000305}. Cytoplasm
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the DENND6 family. {ECO:0000305}.
CC -!- CAUTION: Identified as having similarity to the core DENN family and
CC referred to as DENN6B. Prediction methods do not indicate a DENN domain
CC for this sequence and, the exact role of the DENN or this DENN-like
CC domain in GEF activity needs to be clarified. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB24583.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK006427; BAB24583.1; ALT_INIT; mRNA.
DR EMBL; AK154949; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; CH466550; EDL04366.1; -; Genomic_DNA.
DR EMBL; BC139003; AAI39004.1; -; mRNA.
DR CCDS; CCDS57000.1; -.
DR RefSeq; NP_081357.2; NM_027081.3.
DR AlphaFoldDB; Q9D9V7; -.
DR STRING; 10090.ENSMUSP00000077978; -.
DR iPTMnet; Q9D9V7; -.
DR PhosphoSitePlus; Q9D9V7; -.
DR MaxQB; Q9D9V7; -.
DR PaxDb; Q9D9V7; -.
DR PeptideAtlas; Q9D9V7; -.
DR PRIDE; Q9D9V7; -.
DR ProteomicsDB; 279623; -.
DR Antibodypedia; 6832; 49 antibodies from 16 providers.
DR DNASU; 69440; -.
DR Ensembl; ENSMUST00000078953; ENSMUSP00000077978; ENSMUSG00000015377.
DR GeneID; 69440; -.
DR KEGG; mmu:69440; -.
DR UCSC; uc029suk.1; mouse.
DR CTD; 414918; -.
DR MGI; MGI:1916690; Dennd6b.
DR VEuPathDB; HostDB:ENSMUSG00000015377; -.
DR eggNOG; KOG2432; Eukaryota.
DR GeneTree; ENSGT00390000005529; -.
DR HOGENOM; CLU_017013_0_1_1; -.
DR InParanoid; Q9D9V7; -.
DR OMA; QLVYPYD; -.
DR OrthoDB; 252077at2759; -.
DR PhylomeDB; Q9D9V7; -.
DR TreeFam; TF320228; -.
DR Reactome; R-MMU-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR BioGRID-ORCS; 69440; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Dennd6b; mouse.
DR PRO; PR:Q9D9V7; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9D9V7; protein.
DR Bgee; ENSMUSG00000015377; Expressed in retinal neural layer and 140 other tissues.
DR ExpressionAtlas; Q9D9V7; baseline and differential.
DR Genevisible; Q9D9V7; MM.
DR GO; GO:0055037; C:recycling endosome; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
DR InterPro; IPR018307; ABL9/DENND6_dom.
DR InterPro; IPR024224; DENND6.
DR InterPro; IPR037516; Tripartite_DENN.
DR PANTHER; PTHR13677; PTHR13677; 1.
DR Pfam; PF09794; Avl9; 1.
DR PROSITE; PS50211; DENN; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Endosome; Guanine-nucleotide releasing factor;
KW Reference proteome.
FT CHAIN 1..585
FT /note="Protein DENND6B"
FT /id="PRO_0000264991"
FT DOMAIN 43..221
FT /note="uDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 246..373
FT /note="cDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT DOMAIN 375..499
FT /note="dDENN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00304"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 585 AA; 66588 MW; 512FB9D782E3E252 CRC64;
MEVPVGPGPR QAGGGLGATR SSSSGRAART AEMPWARFSA WLECVCVVTF DLELGQALEL
VYPSDFRLTD KEKSSICYLA FPDSHSGCLG DTQFSFRMRQ CGGQRSLWQV DDRSYNNKAP
LALQREPAHY LGYVYFRQVK DSSVKRGYFQ KSLVLVSRLP FVRLFQSLLS LIAPEYFEKL
APCLEAVCNE IDQWPAPVPG QTLNLPIMGV VIQVRIPSRV DKLESSPPKQ CDQENLLPAP
VVLTSVHELD LFRCFRPVLT HVQTLWELML LGEPLVVLAP SPDVSSELVL ALTSCLQPLK
FCCDFRPYFT IHDSEFKELT TRTQAPPNVV LGVTNPFFIK TLQHWPHVLR IGEPKMSGDL
PKQVKLKKPS RLKTLDTKPG LYTSYTAHLH RDKALLKRLL KGVQKKRPWD VQSALLRRHL
LELTQSFIIP LEHYMASLMP LQKNITPWKS PPQICPFRQD DFLRSLEHAG PQLTCILKGD
WLGLYRRFFK SPHFDGWYRQ RHKEMAQKLE ALHLEAICEA NIEAWMKDKS EVEVVDLVLK
LREKLVRAQG HQLPVKEVTL QRAQLYIDTV IGSLPKDLQA VLCPP
