DEOR_BACSU
ID DEOR_BACSU Reviewed; 313 AA.
AC P39140;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Deoxyribonucleoside regulator {ECO:0000305};
GN Name=deoR {ECO:0000303|PubMed:8550462}; Synonyms=yxxC;
GN OrderedLocusNames=BSU39430;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=168;
RX PubMed=8550462; DOI=10.1128/jb.178.2.424-434.1996;
RA Saxild H.H., Andersen L.N., Hammer K.;
RT "Dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction
RT by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded
RT DeoR repressor protein.";
RL J. Bacteriol. 178:424-434(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BGSC1A1;
RX PubMed=8867804; DOI=10.1093/dnares/2.6.295;
RA Yoshida K., Fujimyra M., Yanai N., Fujita Y.;
RT "Cloning and sequencing of a 23-kb region of the Bacillus subtilis genome
RT between the iol and hut operons.";
RL DNA Res. 2:295-301(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION, AND DNA-BINDING.
RC STRAIN=168;
RX PubMed=10074062; DOI=10.1128/jb.181.6.1719-1727.1999;
RA Zeng X., Saxild H.H.;
RT "Identification and characterization of a DeoR-specific operator sequence
RT essential for induction of dra-nupC-pdp operon expression in Bacillus
RT subtilis.";
RL J. Bacteriol. 181:1719-1727(1999).
RN [5]
RP FUNCTION, DNA-BINDING, AND SUBUNIT.
RC STRAIN=168;
RX PubMed=10714997; DOI=10.1128/jb.182.7.1916-1922.2000;
RA Zeng X., Saxild H.H., Switzer R.L.;
RT "Purification and characterization of the DeoR repressor of Bacillus
RT subtilis.";
RL J. Bacteriol. 182:1916-1922(2000).
CC -!- FUNCTION: Negative regulator of the dra-nupC-pdp operon. DeoR binds
CC cooperatively to the operator DNA, which consists of a palindrome and a
CC direct repeat sequence located 3' to the palindrome.
CC {ECO:0000269|PubMed:10074062, ECO:0000269|PubMed:10714997,
CC ECO:0000269|PubMed:8550462}.
CC -!- SUBUNIT: Homooctamer. {ECO:0000269|PubMed:10714997}.
CC -!- INTERACTION:
CC P39140; P39140: deoR; NbExp=3; IntAct=EBI-9538573, EBI-9538573;
CC -!- MISCELLANEOUS: Deoxyribose-5-phosphate is most likely the effector that
CC modulates binding of DeoR to DNA. {ECO:0000269|PubMed:10074062,
CC ECO:0000269|PubMed:10714997}.
CC -!- SIMILARITY: Belongs to the SorC transcriptional regulatory family.
CC {ECO:0000305}.
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DR EMBL; X82174; CAA57661.1; -; Genomic_DNA.
DR EMBL; D45912; BAA08336.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15979.1; -; Genomic_DNA.
DR PIR; S78768; S49454.
DR RefSeq; NP_391822.1; NC_000964.3.
DR RefSeq; WP_003227124.1; NZ_JNCM01000034.1.
DR PDB; 4OQP; X-ray; 1.60 A; A=56-313.
DR PDB; 4OQQ; X-ray; 1.80 A; A/B=56-313.
DR PDB; 7BHY; X-ray; 2.30 A; A/B/C=4-55.
DR PDBsum; 4OQP; -.
DR PDBsum; 4OQQ; -.
DR PDBsum; 7BHY; -.
DR AlphaFoldDB; P39140; -.
DR SMR; P39140; -.
DR MINT; P39140; -.
DR STRING; 224308.BSU39430; -.
DR PaxDb; P39140; -.
DR PRIDE; P39140; -.
DR EnsemblBacteria; CAB15979; CAB15979; BSU_39430.
DR GeneID; 937543; -.
DR KEGG; bsu:BSU39430; -.
DR PATRIC; fig|224308.179.peg.4268; -.
DR eggNOG; COG2390; Bacteria.
DR InParanoid; P39140; -.
DR OMA; WINGLVT; -.
DR PhylomeDB; P39140; -.
DR BioCyc; BSUB:BSU39430-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR007324; Sugar-bd_dom_put.
DR Pfam; PF04198; Sugar-bind; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
DR SUPFAM; SSF88659; SSF88659; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..313
FT /note="Deoxyribonucleoside regulator"
FT /id="PRO_0000062788"
FT DNA_BIND 23..42
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT HELIX 4..17
FT /evidence="ECO:0007829|PDB:7BHY"
FT HELIX 23..30
FT /evidence="ECO:0007829|PDB:7BHY"
FT HELIX 34..46
FT /evidence="ECO:0007829|PDB:7BHY"
FT STRAND 49..54
FT /evidence="ECO:0007829|PDB:7BHY"
FT HELIX 61..72
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 75..80
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 87..105
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 111..114
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 118..126
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 136..141
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 146..148
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 153..163
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 173..176
FT /evidence="ECO:0007829|PDB:4OQQ"
FT HELIX 180..187
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 190..201
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 203..207
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 217..219
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 220..222
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 226..235
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 238..240
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 243..245
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 254..257
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 265..268
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 271..277
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 281..283
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 284..292
FT /evidence="ECO:0007829|PDB:4OQP"
FT STRAND 297..302
FT /evidence="ECO:0007829|PDB:4OQP"
FT HELIX 303..310
FT /evidence="ECO:0007829|PDB:4OQP"
SQ SEQUENCE 313 AA; 35253 MW; DBBAE7EFFB71471B CRC64;
MDREKQQLSI EAARLYYQSD YSQQQIAEQL NISRPTVSRL LQYAKEKGYV QIRVMDPFED
LDALGSILEE KYGLLEAHVV FSPTPDYAGI THDLSRYGAE YMHETVKDGD IVGVSWGTTM
YQIAQNMQPK QVKGVEVVQL KGGISHSRVN TYSAETIQLF AEAFQTMPRY LPLPVVFDNA
DVKRMVEKDR HIERIIEMGK QANIALFTVG TVRDEALLFR LGYFNEEEKA LLKKQAVGDI
CSRFFDAKGN ICSSAINDRT IGVELQDLRL KERSILVAGG SRKVSSIHGA LTGKYANVLI
IDQHTARALV NDL
