DEP1A_MOUSE
ID DEP1A_MOUSE Reviewed; 804 AA.
AC Q8CIG0; Q8BHF1; Q99JN3;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=DEP domain-containing protein 1A;
GN Name=Depdc1a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Liver, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 532-804 (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 354-364, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
CC -!- FUNCTION: May be involved in transcriptional regulation as a
CC transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a
CC critical role in bladder carcinogenesis by repressing the transcription
CC of the A20 gene, leading to transport of NF-KB protein into the
CC nucleus, resulting in suppression of apoptosis of bladder cancer cells
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Can form dimers. Interacts with ZNF224 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Note=Colocalizes with
CC ZNF224 at the nucleus. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CIG0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CIG0-2; Sequence=VSP_024656, VSP_024657, VSP_024658;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH05799.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK050456; BAC34267.1; -; mRNA.
DR EMBL; AK088719; BAC40526.1; -; mRNA.
DR EMBL; BC005799; AAH05799.1; ALT_INIT; mRNA.
DR EMBL; BC023934; AAH23934.1; -; mRNA.
DR CCDS; CCDS17936.1; -. [Q8CIG0-1]
DR CCDS; CCDS51106.1; -. [Q8CIG0-2]
DR RefSeq; NP_001165563.1; NM_001172092.1.
DR RefSeq; NP_001165564.1; NM_001172093.1. [Q8CIG0-2]
DR RefSeq; NP_083799.2; NM_029523.3. [Q8CIG0-1]
DR AlphaFoldDB; Q8CIG0; -.
DR SMR; Q8CIG0; -.
DR STRING; 10090.ENSMUSP00000113216; -.
DR iPTMnet; Q8CIG0; -.
DR PhosphoSitePlus; Q8CIG0; -.
DR jPOST; Q8CIG0; -.
DR PaxDb; Q8CIG0; -.
DR PRIDE; Q8CIG0; -.
DR ProteomicsDB; 279374; -. [Q8CIG0-1]
DR ProteomicsDB; 279375; -. [Q8CIG0-2]
DR Antibodypedia; 33419; 151 antibodies from 20 providers.
DR DNASU; 76131; -.
DR Ensembl; ENSMUST00000029825; ENSMUSP00000029825; ENSMUSG00000028175. [Q8CIG0-1]
DR Ensembl; ENSMUST00000106041; ENSMUSP00000101656; ENSMUSG00000028175. [Q8CIG0-2]
DR GeneID; 76131; -.
DR KEGG; mmu:76131; -.
DR UCSC; uc008rvy.2; mouse. [Q8CIG0-1]
DR UCSC; uc008rwa.2; mouse. [Q8CIG0-2]
DR CTD; 76131; -.
DR MGI; MGI:1923381; Depdc1a.
DR VEuPathDB; HostDB:ENSMUSG00000028175; -.
DR eggNOG; ENOG502QR00; Eukaryota.
DR GeneTree; ENSGT00950000182976; -.
DR HOGENOM; CLU_040757_0_0_1; -.
DR InParanoid; Q8CIG0; -.
DR OMA; VPQYVMY; -.
DR OrthoDB; 413007at2759; -.
DR PhylomeDB; Q8CIG0; -.
DR BioGRID-ORCS; 76131; 1 hit in 73 CRISPR screens.
DR PRO; PR:Q8CIG0; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8CIG0; protein.
DR Bgee; ENSMUSG00000028175; Expressed in undifferentiated genital tubercle and 53 other tissues.
DR ExpressionAtlas; Q8CIG0; baseline and differential.
DR Genevisible; Q8CIG0; MM.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0017053; C:transcription repressor complex; ISS:UniProtKB.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.555.10; -; 1.
DR InterPro; IPR000591; DEP_dom.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00610; DEP; 1.
DR SMART; SM00049; DEP; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS50186; DEP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Direct protein sequencing;
KW GTPase activation; Nucleus; Phosphoprotein; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..804
FT /note="DEP domain-containing protein 1A"
FT /id="PRO_0000284787"
FT DOMAIN 24..108
FT /note="DEP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00066"
FT DOMAIN 282..322
FT /note="Rho-GAP"
FT REGION 592..647
FT /note="Interaction with ZNF224"
FT /evidence="ECO:0000250"
FT COILED 726..760
FT /evidence="ECO:0000255"
FT MOD_RES 513
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5TB30"
FT VAR_SEQ 305
FT /note="V -> G (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_024656"
FT VAR_SEQ 306..582
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_024657"
FT VAR_SEQ 760
FT /note="Q -> QFQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_024658"
SQ SEQUENCE 804 AA; 92201 MW; C811F34A87EE33D9 CRC64;
MERQGAPLGP YRATKLWNEV TTSFRVGMPL RKHRQHLKKY SNCFTAVEAI DWLYDLLRSN
SNFGPEVTRQ QTIQLLRKFL KNHVIEDIKG RWGSESLDDN NQLFRFPATS PLKTFPQRHT
EIKKTNTEYF SKDKDGIFKL RNLSQKTSKK RGLHFSQENT EKINHERITN EDLEIAPDNQ
EISQEDVEEV WRYVIMIYLQ TILSLPSIEE LLNPNQVIPQ YIMYNMANTS KHGVVILQDK
SDDLPHWVLS AMKCLANWPR SNDTNNLTYV GFERDVFKTI ADYFLNLPEP LLTFEYYELF
VNILVVCGYI TVSDRTSGIH KIQDDPRSSK IHDLSNLNSF KSTECLLLSL LYKDKSNEEL
DSTKRLQRND QGFQERCAKK MQLDNLRNRR ASANDIMGGS CHNLIGLSNT NALSSNIKPR
CSSLEGIVDR PVNSSEKKSS IFYQSVLNIE EQNSKQSLVS APKQTPLFNL HSDENAQQPH
CVGFNRTSAL TVQDQEELCN EKYKSKQLCR SQSLLLRSST RQNSCINKPV AEIIMKPNVG
QGSTSELGES STTINKRLCK STIELSEKSL PPAASVLTGT QSLLQPHLER VAINALQLCC
LLLPPPNRRK LQLLMRMISR MSQNVDMPKL HEQIGTRSLM INTFSRCVLC CAEEVDLDEL
LASRLVSFLM DHHQEILQVP TYLQAAVEKH LDYIKKGNVK NHGDGLVVPL PTYSYCKQIS
AKEFDEQKIS TSQAAIAELL ENIVRSKSLS LKEKRRKLKQ KEYPLIYQKR FPTTESEAAL
FDDKPTIKQP MLNLRNPKLH SLRY
