DEP1B_HUMAN
ID DEP1B_HUMAN Reviewed; 529 AA.
AC Q8WUY9; A8K3R9; B4DUT4; Q86WJ3; Q8IZY6; Q9NUN3; Q9NW57;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-APR-2007, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=DEP domain-containing protein 1B;
DE AltName: Full=HBV X-transactivated gene 8 protein;
DE AltName: Full=HBV XAg-transactivated protein 8;
GN Name=DEPDC1B; Synonyms=XTP8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Mammary cancer;
RA Gokhale P.C., Kumar D., Monia B.P., Rahman A., Dritschilo A., Kasid U.;
RT "Novel transcript in human breast cancer cells.";
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND VARIANTS MET-332 AND LEU-395.
RA Liu Y., Cheng J., Lu Y., Wang G., Zhang L., Chen J., Li L.;
RT "Cloning and identification of human gene 8 transactivated by hepatitis B
RT virus X antigen.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS MET-332
RP AND LEU-395.
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160 AND SER-436, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8WUY9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8WUY9-2; Sequence=VSP_042922;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH10904.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAA91529.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF303178; AAN31126.1; -; mRNA.
DR EMBL; AF488828; AAO49477.1; -; mRNA.
DR EMBL; AF490257; AAO85465.1; -; mRNA.
DR EMBL; AK001166; BAA91529.1; ALT_INIT; mRNA.
DR EMBL; AK002114; BAA92089.1; -; mRNA.
DR EMBL; AK290684; BAF83373.1; -; mRNA.
DR EMBL; AK300782; BAG62446.1; -; mRNA.
DR EMBL; AC016591; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC109133; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471123; EAW54998.1; -; Genomic_DNA.
DR EMBL; BC010904; AAH10904.1; ALT_INIT; mRNA.
DR EMBL; BC019075; AAH19075.1; -; mRNA.
DR CCDS; CCDS3977.1; -. [Q8WUY9-1]
DR CCDS; CCDS47214.1; -. [Q8WUY9-2]
DR RefSeq; NP_001138680.1; NM_001145208.1. [Q8WUY9-2]
DR RefSeq; NP_060839.2; NM_018369.2. [Q8WUY9-1]
DR AlphaFoldDB; Q8WUY9; -.
DR SMR; Q8WUY9; -.
DR BioGRID; 120903; 176.
DR IntAct; Q8WUY9; 37.
DR MINT; Q8WUY9; -.
DR STRING; 9606.ENSP00000265036; -.
DR iPTMnet; Q8WUY9; -.
DR PhosphoSitePlus; Q8WUY9; -.
DR BioMuta; DEPDC1B; -.
DR DMDM; 145558895; -.
DR EPD; Q8WUY9; -.
DR jPOST; Q8WUY9; -.
DR MassIVE; Q8WUY9; -.
DR MaxQB; Q8WUY9; -.
DR PaxDb; Q8WUY9; -.
DR PeptideAtlas; Q8WUY9; -.
DR PRIDE; Q8WUY9; -.
DR ProteomicsDB; 74728; -. [Q8WUY9-1]
DR ProteomicsDB; 74729; -. [Q8WUY9-2]
DR Antibodypedia; 23636; 175 antibodies from 23 providers.
DR DNASU; 55789; -.
DR Ensembl; ENST00000265036.10; ENSP00000265036.5; ENSG00000035499.13. [Q8WUY9-1]
DR Ensembl; ENST00000453022.6; ENSP00000389101.2; ENSG00000035499.13. [Q8WUY9-2]
DR GeneID; 55789; -.
DR KEGG; hsa:55789; -.
DR MANE-Select; ENST00000265036.10; ENSP00000265036.5; NM_018369.3; NP_060839.2.
DR UCSC; uc003jsh.4; human. [Q8WUY9-1]
DR CTD; 55789; -.
DR DisGeNET; 55789; -.
DR GeneCards; DEPDC1B; -.
DR HGNC; HGNC:24902; DEPDC1B.
DR HPA; ENSG00000035499; Tissue enhanced (gallbladder, lymphoid tissue, testis).
DR MIM; 616066; gene.
DR neXtProt; NX_Q8WUY9; -.
DR OpenTargets; ENSG00000035499; -.
DR PharmGKB; PA134941087; -.
DR VEuPathDB; HostDB:ENSG00000035499; -.
DR eggNOG; ENOG502QR00; Eukaryota.
DR GeneTree; ENSGT00950000182976; -.
DR HOGENOM; CLU_040757_0_0_1; -.
DR InParanoid; Q8WUY9; -.
DR OMA; ELPHWIL; -.
DR OrthoDB; 413007at2759; -.
DR PhylomeDB; Q8WUY9; -.
DR TreeFam; TF328365; -.
DR PathwayCommons; Q8WUY9; -.
DR Reactome; R-HSA-8980692; RHOA GTPase cycle.
DR Reactome; R-HSA-9013026; RHOB GTPase cycle.
DR Reactome; R-HSA-9013106; RHOC GTPase cycle.
DR Reactome; R-HSA-9013148; CDC42 GTPase cycle.
DR Reactome; R-HSA-9013149; RAC1 GTPase cycle.
DR Reactome; R-HSA-9013404; RAC2 GTPase cycle.
DR Reactome; R-HSA-9013405; RHOD GTPase cycle.
DR Reactome; R-HSA-9013406; RHOQ GTPase cycle.
DR Reactome; R-HSA-9013408; RHOG GTPase cycle.
DR Reactome; R-HSA-9013409; RHOJ GTPase cycle.
DR Reactome; R-HSA-9013420; RHOU GTPase cycle.
DR Reactome; R-HSA-9013423; RAC3 GTPase cycle.
DR Reactome; R-HSA-9013424; RHOV GTPase cycle.
DR Reactome; R-HSA-9035034; RHOF GTPase cycle.
DR Reactome; R-HSA-9696264; RND3 GTPase cycle.
DR Reactome; R-HSA-9696270; RND2 GTPase cycle.
DR Reactome; R-HSA-9696273; RND1 GTPase cycle.
DR SignaLink; Q8WUY9; -.
DR BioGRID-ORCS; 55789; 12 hits in 1081 CRISPR screens.
DR ChiTaRS; DEPDC1B; human.
DR GenomeRNAi; 55789; -.
DR Pharos; Q8WUY9; Tbio.
DR PRO; PR:Q8WUY9; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q8WUY9; protein.
DR Bgee; ENSG00000035499; Expressed in secondary oocyte and 124 other tissues.
DR ExpressionAtlas; Q8WUY9; baseline and differential.
DR Genevisible; Q8WUY9; HS.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0016477; P:cell migration; IMP:UniProtKB.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0030177; P:positive regulation of Wnt signaling pathway; IMP:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.555.10; -; 1.
DR InterPro; IPR000591; DEP_dom.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00610; DEP; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00049; DEP; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR PROSITE; PS50186; DEP; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; GTPase activation; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..529
FT /note="DEP domain-containing protein 1B"
FT /id="PRO_0000284790"
FT DOMAIN 24..108
FT /note="DEP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00066"
FT DOMAIN 201..393
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT MOD_RES 160
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 436
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 414..475
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_042922"
FT VARIANT 332
FT /note="R -> M (in dbSNP:rs17856590)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.2"
FT /id="VAR_031819"
FT VARIANT 395
FT /note="V -> L (in dbSNP:rs17851707)"
FT /evidence="ECO:0000269|PubMed:15489334, ECO:0000269|Ref.2"
FT /id="VAR_031820"
FT CONFLICT 272
FT /note="F -> C (in Ref. 1; AAN31126 and 3; BAA92089)"
FT /evidence="ECO:0000305"
FT CONFLICT 380
FT /note="R -> K (in Ref. 1; AAN31126)"
FT /evidence="ECO:0000305"
FT CONFLICT 402
FT /note="S -> P (in Ref. 3; BAA92089)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 529 AA; 61771 MW; E640F8034BD308C6 CRC64;
MEHRIVGPGP YRATRLWNET VELFRAKMPL RKHRCRFKSY EHCFTAAEAV DWLHELLRCS
QNFGPEVTRK QTVQLLKKFL KNHVIEDIKG KWGEEDFEDN RHLYRFPPSS PLKPYPKKPP
NQKDVIKFPE WNDLPPGTSQ ENIPVRPVVM NSEMWYKRHS IAIGEVPACR LVHRRQLTEA
NVEEIWKSMT LSYLQKILGL DSLEEVLDVK LVNSKFIIHN VYSVSKQGVV ILDDKSKELP
HWVLSAMKCL ANWPNCSDLK QPMYLGFEKD VFKTIADYYG HLKEPLLTFH LFDAFVSVLG
LLQKEKVAVE AFQICCLLLP PENRRKLQLL MRMMARICLN KEMPPLCDGF GTRTLMVQTF
SRCILCSKDE VDLDELLAAR LVTFLMDNYQ EILKVPLALQ TSIEERVAHL RRVQIKYPGA
DMDITLSAPS FCRQISPEEF EYQRSYGSQE PLAALLEEVI TDAKLSNKEK KKKLKQFQKS
YPEVYQERFP TPESAALLFP EKPKPKPQLL MWALKKPFQP FQRTRSFRM
