ID   DEP1_ALTBR              Reviewed;         369 AA.
AC   D2E9W6;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   09-FEB-2010, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=Terpene cyclase DEP1 {ECO:0000250|UniProtKB:A0A455R4Z0};
DE            EC=5.4.99.- {ECO:0000250|UniProtKB:A0A455R4Z0};
DE   AltName: Full=Depudecin biosynthesis cluster protein 1 {ECO:0000303|PubMed:19737099};
GN   Name=DEP1 {ECO:0000303|PubMed:19737099};
OS   Alternaria brassicicola (Dark leaf spot agent).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Alternaria;
OC   Alternaria sect. Brassicicola.
OX   NCBI_TaxID=29001;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, AND PATHWAY.
RC   STRAIN=MUCL 202097;
RX   PubMed=19737099; DOI=10.1094/mpmi-22-10-1258;
RA   Wight W.D., Kim K.-H., Lawrence C.B., Walton J.D.;
RT   "Biosynthesis and role in virulence of the histone deacetylase inhibitor
RT   depudecin from Alternaria brassicicola.";
RL   Mol. Plant Microbe Interact. 22:1258-1267(2009).
CC   -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC       depudecin, a highly oxidized eleven-carbon linear polyketide that acts
CC       as a histone deacetylase (HDAC) inhibitor and makes a small
CC       contribution to pathogenesis (PubMed:19737099). The reducing polyketide
CC       synthase DEP5 is the central enzyme in depudecin biosynthesis by
CC       yielding the backbone polyketide chain (PubMed:19737099). The
CC       monooxygenases DEP2 and DEP4, as well as the uncharacterized protein
CC       DEP1, then act as tailoring enzymes to modify the intermediate
CC       polyketide chain into depudecin (PubMed:19737099).
CC       {ECO:0000269|PubMed:19737099}.
CC   -!- PATHWAY: Polyketide biosynthesis. {ECO:0000269|PubMed:19737099}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is positively regulated by the depudecin
CC       biosynthesis cluster-specific transcription activator DEP6
CC       (PubMed:19737099). {ECO:0000269|PubMed:19737099}.
CC   -!- SIMILARITY: Belongs to the membrane-bound ascI terpene cyclase family.
CC       {ECO:0000305}.
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DR   EMBL; FJ977165; ACZ57550.1; -; Genomic_DNA.
DR   AlphaFoldDB; D2E9W6; -.
DR   PHI-base; PHI:2375; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Isomerase; Membrane; Transmembrane; Transmembrane helix.
FT   CHAIN           1..369
FT                   /note="Terpene cyclase DEP1"
FT                   /id="PRO_0000441935"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        82..102
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        157..177
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        190..210
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        234..254
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        298..318
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        342..362
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   369 AA;  40287 MW;  F9C801674A51DF92 CRC64;
     MPQTSTTQFF YLSALGIWGL WVYAFFNGMF DRLDTITRTL HFPDGRPLRS KYTNIGPLDA
     QLTLLSAFYD VLSNTLTSGP RLLFFDVNYV VACANLWVLI ESRRRGVRSW FLKYPAWAMV
     LCNANGAAIV LPLYLYLVCC SKARLRDASV PKHEATALLV STVVILLQPL LIFVPAWAGR
     GGSHLHHGCI ALFQVAPIGV SVFHLGLASI LPREASDSSP SSRKDSKKCI VASLVLAGTV
     AAAVHSYTVV GALITRDGQA SLTRLFVPAH GFSDPIEVPL QPSGLPAEYM ALVENLHLFS
     QWDWIVVALT SVLYSHLLLS LRDGAVRAKA NHWVSPVEAQ ELVYLTVATI ILGPGGAASF
     ALAIRESRI