ID   DEP3_ALTBR              Reviewed;         564 AA.
AC   D2E9W8;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   09-FEB-2010, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Efflux pump DEP3 {ECO:0000303|PubMed:19737099};
DE   AltName: Full=Depudecin biosynthesis cluster protein 3 {ECO:0000303|PubMed:19737099};
GN   Name=DEP3 {ECO:0000303|PubMed:19737099};
OS   Alternaria brassicicola (Dark leaf spot agent).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Alternaria;
OC   Alternaria sect. Brassicicola.
OX   NCBI_TaxID=29001;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC   STRAIN=MUCL 202097;
RX   PubMed=19737099; DOI=10.1094/mpmi-22-10-1258;
RA   Wight W.D., Kim K.-H., Lawrence C.B., Walton J.D.;
RT   "Biosynthesis and role in virulence of the histone deacetylase inhibitor
RT   depudecin from Alternaria brassicicola.";
RL   Mol. Plant Microbe Interact. 22:1258-1267(2009).
CC   -!- FUNCTION: Efflux pump; part of the gene cluster that mediates the
CC       biosynthesis of depudecin, a highly oxidized eleven-carbon linear
CC       polyketide that acts as a histone deacetylase (HDAC) inhibitor and
CC       makes a small contribution to pathogenesis (PubMed:19737099). Is
CC       presumed either to be responsible for exporting depudecin, to provide
CC       self-protection, or both (PubMed:19737099).
CC       {ECO:0000269|PubMed:19737099}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is positively regulated by the depudecin
CC       biosynthesis cluster-specific transcription activator DEP6
CC       (PubMed:19737099). {ECO:0000269|PubMed:19737099}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; FJ977165; ACZ57546.1; -; Genomic_DNA.
DR   AlphaFoldDB; D2E9W8; -.
DR   SMR; D2E9W8; -.
DR   PHI-base; PHI:2377; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 2.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..564
FT                   /note="Efflux pump DEP3"
FT                   /id="PRO_0000441939"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        122..142
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        212..232
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        255..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        281..301
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        362..382
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        386..406
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        423..443
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        452..472
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        528..548
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   564 AA;  60157 MW;  35ECC9643A427C05 CRC64;
     MVYSSTSSSQ NRPDGEKHVE AVGSSTRIPS DELVDRGGGD TTTGKQSPRD IHGWKWATAY
     SAMLSTTLLF ALDNTIVANI QPAIINDFGH LELLSWIGTG FALGTMFILL WGKVYGVFNI
     KWVYIFNIFL FEAGSALCGA APNMAALIIG RVIAGVGGSG MYSGTLTYVS VLSNDQEKPA
     YLAGSTVVWG IGSVLGPVVG GAFAASSATW RWGFYVNLPI GAAFAPVYFL LFPSFDPYPS
     KTLAEKLRLV DWINAVIFLA GSACLTVVLT FGGVVYPFNS GTVIALWTVT GILLVAFIVL
     LKLHPLVAKE NRLYPLHFFK QPTLINMQLQ VFLSSGIILA MTYYVPLYFQ FIKGDGALEA
     GVRLLPLIMF MVVASMVNGF LMPRYGLIPI WYIGGSSLAL IGTALMYTVD DSTSNANIYG
     YNILIGAGTG SYIVAGFAIV QSLVPVHEIA NAVGAMTIFQ DLGMVLFLAI SGSLFHNVAV
     DKVGKALPDV SHTEIANLIA GSSSKAFQAL SDTEKALVIP EIASAMTTIW AFFLAAAALS
     VVCSFPLLKA KIGGMEKRTA ITAA