ID   DERF6_DERFA             Reviewed;         279 AA.
AC   P49276; Q9NJS0;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   20-JUN-2001, sequence version 2.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Mite allergen Der f 6;
DE            EC=3.4.21.-;
DE   AltName: Full=Allergen Der f VI;
DE   AltName: Full=DF5;
DE   AltName: Allergen=Der f 6;
DE   Flags: Precursor;
GN   Name=DERF6;
OS   Dermatophagoides farinae (American house dust mite).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Acariformes; Sarcoptiformes; Astigmata; Psoroptidia; Analgoidea;
OC   Pyroglyphidae; Dermatophagoidinae; Dermatophagoides.
OX   NCBI_TaxID=6954;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10381565;
RA   Kawamoto S., Mizuguchi Y., Morimoto K., Aki T., Shigeta S., Yasueda H.,
RA   Wada T., Suzuki O., Jyo T., Ono K.;
RT   "Cloning and expression of Der f 6, a serine protease allergen from the
RT   house dust mite, Dermatophagoides farinae.";
RL   Biochim. Biophys. Acta 1454:201-207(1999).
RN   [2]
RP   PROTEIN SEQUENCE OF 50-69.
RX   PubMed=8334537; DOI=10.1111/j.1365-2222.1993.tb00343.x;
RA   Yasueda H., Mita H., Akiyama K., Shida T., Ando T., Sugiyama S.,
RA   Yamakawa H.;
RT   "Allergens from Dermatophagoides mites with chymotryptic activity.";
RL   Clin. Exp. Allergy 23:384-390(1993).
CC   -!- FUNCTION: Protease that shows specificity similar to chymotrypsin.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Common symptoms of mite
CC       allergy are bronchial asthma, allergic rhinitis and conjunctivitis.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00274}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF125187; AAF28423.1; -; mRNA.
DR   AlphaFoldDB; P49276; -.
DR   SMR; P49276; -.
DR   Allergome; 306; Der f 6.
DR   Allergome; 3255; Der f 6.0101.
DR   MEROPS; S01.245; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; -; 1.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR018114; TRYPSIN_HIS.
DR   InterPro; IPR033116; TRYPSIN_SER.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
PE   1: Evidence at protein level;
KW   Allergen; Direct protein sequencing; Disulfide bond; Hydrolase; Protease;
KW   Secreted; Serine protease; Signal; Zymogen.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..49
FT                   /evidence="ECO:0000269|PubMed:8334537"
FT                   /id="PRO_0000028147"
FT   CHAIN           50..279
FT                   /note="Mite allergen Der f 6"
FT                   /id="PRO_0000028148"
FT   DOMAIN          50..279
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        94
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        140
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        233
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   DISULFID        79..95
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        204..220
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        229..255
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   CONFLICT        50
FT                   /note="V -> A (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   279 AA;  30535 MW;  5B29B6C46F185E56 CRC64;
     MIKIFLVTIL IVITVTVDAR FPRSLQPKWA YLDSNEFPRS KIGDSPIAGV VGGQDADLAE
     APFQISLLKD YLIMKRHMCG GSLISESTVV TAAHCTYGQK ASSLSVRYGT NQRTSSSYGD
     LKVKPIIQHE SYEQDQTQTD KTIIILPNPV VPSTNVQMNE IETEDIVDGD KVTIYGWGLT
     DGNGKDLPDK LQKGSMTIVG NDRCNEKWGS INAIHPGMIC ALDKTQSGCN GDSGGPLVSA
     NRKLTGIVSW GPSKCPPGEY MSVFTRPKYY LDWITKNIV