DER_COXBU
ID DER_COXBU Reviewed; 443 AA.
AC Q83C83;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=GTPase Der {ECO:0000255|HAMAP-Rule:MF_00195};
DE AltName: Full=GTP-binding protein EngA {ECO:0000255|HAMAP-Rule:MF_00195};
GN Name=der {ECO:0000255|HAMAP-Rule:MF_00195}; Synonyms=engA;
GN OrderedLocusNames=CBU_1245;
OS Coxiella burnetii (strain RSA 493 / Nine Mile phase I).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae;
OC Coxiella.
OX NCBI_TaxID=227377;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RSA 493 / Nine Mile phase I;
RX PubMed=12704232; DOI=10.1073/pnas.0931379100;
RA Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C.,
RA Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T.,
RA Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M.,
RA Lee K.H., Carty H.A., Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E.,
RA Fraser C.M., Heidelberg J.F.;
RT "Complete genome sequence of the Q-fever pathogen, Coxiella burnetii.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003).
CC -!- FUNCTION: GTPase that plays an essential role in the late steps of
CC ribosome biogenesis. {ECO:0000255|HAMAP-Rule:MF_00195}.
CC -!- SUBUNIT: Associates with the 50S ribosomal subunit. {ECO:0000255|HAMAP-
CC Rule:MF_00195}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. EngA (Der) GTPase family. {ECO:0000255|HAMAP-
CC Rule:MF_00195}.
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DR EMBL; AE016828; AAO90754.1; -; Genomic_DNA.
DR RefSeq; NP_820240.1; NC_002971.3.
DR RefSeq; WP_005770802.1; NC_002971.4.
DR PDB; 5DN8; X-ray; 1.76 A; A=1-443.
DR PDBsum; 5DN8; -.
DR AlphaFoldDB; Q83C83; -.
DR SMR; Q83C83; -.
DR STRING; 227377.CBU_1245; -.
DR EnsemblBacteria; AAO90754; AAO90754; CBU_1245.
DR GeneID; 1209150; -.
DR KEGG; cbu:CBU_1245; -.
DR PATRIC; fig|227377.7.peg.1237; -.
DR eggNOG; COG1160; Bacteria.
DR HOGENOM; CLU_016077_6_2_6; -.
DR OMA; KFRFLEY; -.
DR Proteomes; UP000002671; Chromosome.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR Gene3D; 3.30.300.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00195; GTPase_Der; 1.
DR InterPro; IPR031166; G_ENGA.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR016484; GTP-bd_EngA.
DR InterPro; IPR032859; KH_dom-like.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR Pfam; PF14714; KH_dom-like; 1.
DR Pfam; PF01926; MMR_HSR1; 2.
DR PIRSF; PIRSF006485; GTP-binding_EngA; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR03594; GTPase_EngA; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 2.
DR PROSITE; PS51712; G_ENGA; 2.
PE 1: Evidence at protein level;
KW 3D-structure; GTP-binding; Nucleotide-binding; Reference proteome; Repeat;
KW Ribosome biogenesis.
FT CHAIN 1..443
FT /note="GTPase Der"
FT /id="PRO_0000178989"
FT DOMAIN 3..167
FT /note="EngA-type G 1"
FT DOMAIN 176..349
FT /note="EngA-type G 2"
FT DOMAIN 350..434
FT /note="KH-like"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 9..16
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 56..60
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 119..122
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 182..189
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 229..233
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT BINDING 294..297
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00195"
FT STRAND 4..8
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 15..22
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 30..33
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 40..47
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 50..56
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 57..59
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 74..82
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 84..91
FT /evidence="ECO:0007829|PDB:5DN8"
FT TURN 92..94
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 98..109
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 110..119
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 126..130
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 131..136
FT /evidence="ECO:0007829|PDB:5DN8"
FT TURN 146..148
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 152..161
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 176..181
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 188..195
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 201..203
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 215..220
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 223..228
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 233..239
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 248..257
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 259..266
FT /evidence="ECO:0007829|PDB:5DN8"
FT TURN 267..269
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 273..285
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 288..294
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 301..314
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 316..318
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 321..325
FT /evidence="ECO:0007829|PDB:5DN8"
FT TURN 328..330
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 334..336
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 337..349
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 354..367
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 379..387
FT /evidence="ECO:0007829|PDB:5DN8"
FT TURN 388..391
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 392..399
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 400..402
FT /evidence="ECO:0007829|PDB:5DN8"
FT HELIX 405..419
FT /evidence="ECO:0007829|PDB:5DN8"
FT STRAND 427..431
FT /evidence="ECO:0007829|PDB:5DN8"
SQ SEQUENCE 443 AA; 49707 MW; 040B36345FD487EF CRC64;
MLPVIAIVGR PNVGKSTLFN YLTKSRAALV ADVPGVTRDR QYGETTIDSQ RLLLVDTGGL
VDTENKEVAP LAETQVEQAI DESDCILFLV DAKAGLVPAD EIIAERLRKK GKKIFLAVNK
ADRARAAVVQ SDFYKLGFGE PYVIAAASGR GVKDLMTQVL ENLPEEKEVI EKEVGIKIAM
IGRPNVGKST LINRLLGEER VIVYDQPGTT RDSIYIPFAR NDENYTLIDT AGIRRRAKIQ
DYVEKFSMIK SLQAMHAADV VIFLLDARQG VTEQDLRLLN RIVEAGVSLI IAVNKWDGLN
IEERDNVRNA IDRRMPFVDF ARRYFISALH GTGVGKLFRA IQESYQSIQQ ELTTGQLTRA
LEKAVAEHEP PLVKGRRIRL RYAHLGARHP LTIVVHGKQT KSLPQSYSRY LANYFRKTFN
FIGVPVHIKL KTDPNPYEGQ EER
